LOCUS       pOME0004      1358 bp    DNA     linear   UNA 14-Oct-2013
DEFINITION  KSHV ORF 10 tagged; 154-4; vector pDEST47
SOURCE      KSHV
  ORGANISM  KSHV
            KSHV ORF 10 tagged; 154-4; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..1341
                     /cds_type=ORF
                     /note="Length: 1323"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            join(115..120,115^116)
                     /site_type="restriction site"
                     /note="Name: BglII"
                     /note="Pattern: agatct"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 115^116"
                     /note="cut_0_on_negative_strand: 119^120"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BglII
     Site            1273..1338
                     /site_type="mutagenized site"
                     /label="3x FLAG tag"
     Site            join(1309..1314,1311^1312)
                     /site_type="restriction site"
                     /note="Name: EcoRV"
                     /note="Pattern: gatatc"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 1311^1312"
                     /note="cut_0_on_negative_strand: 1311^1312"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=EcoRV
     Site            1339..1344
                     /site_type="mutagenized site"
                     /label="Double stop"
     Site            join(1345..1350,1345^1346)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 1345^1346"
                     /note="cut_0_on_negative_strand: 1349^1350"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            1345..1358
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(1351..1358,1352^1353)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 1352^1353"
                     /note="cut_0_on_negative_strand: 1356^1357"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccat gcagacagag gcaacgttca tcctaggtga ctgggagata
       61 acggtgtcta actgccggtt tacttgcagc agcctaacat gtggccccct ttacagatct
      121 agcggcgact acacgcggct aagaatcccc ttctctctgg atcgactaat acgtgaccat
      181 gccatctttg ggctagtgcc aaatattgag gatctgttaa cccatgggtc atgcgtcgcc
      241 gtagtggccg acgcaaacgc cacaggcggc aacgcgcgac gcatcgtcgc gcctggcgtg
      301 ataaacaatt tttcagaacc catcggcatt tgggtacgcg gccctccgcc gcaaacgcgc
      361 aaggaagcta ttaagttctg catatttttt gtcagtccca tgcccccgcg ggagatgacc
      421 acatatgtgt tcaagggcgg cgatttgcct cccggagcag aggaacccga aacactacac
      481 tccgccgagg cacccctacc gtcgcgcgag acgctggtaa ctggacagct gcgatccacc
      541 tcgccgcgaa cgtatacggg atactttcac agtcctgtcc cgctctcttt tttggacctc
      601 ctgacattcg agtccattgg gtgtgacaac gtggaaggtg accccgagca attgacaccc
      661 aagtacttga cgttcacgca gacgggagaa agactttgca aagtaaccgt ttacaacacc
      721 cattcgacag catgcaagaa ggcccgtgtt cgtttcgtct acagaccgac gccgtccgcc
      781 cgtcagcttg tcatgggtca ggcttcaccc ctcataacaa cccctctggg agccagggta
      841 ttcgcagtct atccagactg tgagaaaact atcccacctc aggaaaccac caccctgagg
      901 attcaattgc tgttcgagca gcatggtgcc aacgccggag actgcgcctt tgtcatcatg
      961 gggctcgccc gtgaaacaaa gtttgtctca tttcccgcag tactccttcc gggcaagcac
     1021 gaacacctta ttgtattcaa cccacagaca catcctctga ccattcaacg ggacacaata
     1081 gtgggcgtgg caatggcttg ctatatccac cccggtaagg cagccagcca ggcaccatac
     1141 agcttctacg actgcaagga agagagctgg cacgtggggc tcttccagat caaacgcgga
     1201 ccgggagggg tctgtacacc accttgccac gtagcgatta gggccgaccg ccacgaggaa
     1261 cccatgcaat cggactacaa agaccatgac ggtgattata aagatcatga tatcgattac
     1321 aaggatgacg atgacaagta gtaactcgag gcggccgc
//