LOCUS pOME0004 1358 bp DNA linear UNA 14-Oct-2013 DEFINITION KSHV ORF 10 tagged; 154-4; vector pDEST47 SOURCE KSHV ORGANISM KSHV KSHV ORF 10 tagged; 154-4; Synthetic Construct. REFERENCE 1 (bases 1 to 887) AUTHORS Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P., Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y., Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and Texier,J.R. TITLE Unpublished COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of the Orfeome project FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..1341 /cds_type=ORF /note="Length: 1323" /note="Found at strand: positive" /note="Start codon: atg" Site join(115..120,115^116) /site_type="restriction site" /note="Name: BglII" /note="Pattern: agatct" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 115^116" /note="cut_0_on_negative_strand: 119^120" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=BglII Site 1273..1338 /site_type="mutagenized site" /label="3x FLAG tag" Site join(1309..1314,1311^1312) /site_type="restriction site" /note="Name: EcoRV" /note="Pattern: gatatc" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 1311^1312" /note="cut_0_on_negative_strand: 1311^1312" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=EcoRV Site 1339..1344 /site_type="mutagenized site" /label="Double stop" Site join(1345..1350,1345^1346) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 1345^1346" /note="cut_0_on_negative_strand: 1349^1350" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 1345..1358 /site_type="mutagenized site" /label="3' RE tag" Site join(1351..1358,1352^1353) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 1352^1353" /note="cut_0_on_negative_strand: 1356^1357" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccat gcagacagag gcaacgttca tcctaggtga ctgggagata 61 acggtgtcta actgccggtt tacttgcagc agcctaacat gtggccccct ttacagatct 121 agcggcgact acacgcggct aagaatcccc ttctctctgg atcgactaat acgtgaccat 181 gccatctttg ggctagtgcc aaatattgag gatctgttaa cccatgggtc atgcgtcgcc 241 gtagtggccg acgcaaacgc cacaggcggc aacgcgcgac gcatcgtcgc gcctggcgtg 301 ataaacaatt tttcagaacc catcggcatt tgggtacgcg gccctccgcc gcaaacgcgc 361 aaggaagcta ttaagttctg catatttttt gtcagtccca tgcccccgcg ggagatgacc 421 acatatgtgt tcaagggcgg cgatttgcct cccggagcag aggaacccga aacactacac 481 tccgccgagg cacccctacc gtcgcgcgag acgctggtaa ctggacagct gcgatccacc 541 tcgccgcgaa cgtatacggg atactttcac agtcctgtcc cgctctcttt tttggacctc 601 ctgacattcg agtccattgg gtgtgacaac gtggaaggtg accccgagca attgacaccc 661 aagtacttga cgttcacgca gacgggagaa agactttgca aagtaaccgt ttacaacacc 721 cattcgacag catgcaagaa ggcccgtgtt cgtttcgtct acagaccgac gccgtccgcc 781 cgtcagcttg tcatgggtca ggcttcaccc ctcataacaa cccctctggg agccagggta 841 ttcgcagtct atccagactg tgagaaaact atcccacctc aggaaaccac caccctgagg 901 attcaattgc tgttcgagca gcatggtgcc aacgccggag actgcgcctt tgtcatcatg 961 gggctcgccc gtgaaacaaa gtttgtctca tttcccgcag tactccttcc gggcaagcac 1021 gaacacctta ttgtattcaa cccacagaca catcctctga ccattcaacg ggacacaata 1081 gtgggcgtgg caatggcttg ctatatccac cccggtaagg cagccagcca ggcaccatac 1141 agcttctacg actgcaagga agagagctgg cacgtggggc tcttccagat caaacgcgga 1201 ccgggagggg tctgtacacc accttgccac gtagcgatta gggccgaccg ccacgaggaa 1261 cccatgcaat cggactacaa agaccatgac ggtgattata aagatcatga tatcgattac 1321 aaggatgacg atgacaagta gtaactcgag gcggccgc //