LOCUS       pOME0005R       647 bp    DNA     linear   UNA 14-Oct-2013
DEFINITION  KSHV ORF K2; UNC154-5; vector pVR21
SOURCE      KSHV
  ORGANISM  KSHV
            KSHV ORF K2; UNC154-5; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz 
            pOME= plasmid of the Orfeome project
            "R" in name = in replicon vector= pVR21 
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..633
                     /cds_type=ORF
                     /note="Length: 615"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            join(207..212,207^208)
                     /site_type="restriction site"
                     /note="Name: XbaI"
                     /note="Pattern: tctaga"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 207^208"
                     /note="cut_0_on_negative_strand: 211^212"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XbaI
     Site            join(325..330,325^326)
                     /site_type="restriction site"
                     /note="Name: EcoRI"
                     /note="Pattern: gaattc"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 325^326"
                     /note="cut_0_on_negative_strand: 329^330"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=EcoRI
     Site            join(634..639,634^635)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 634^635"
                     /note="cut_0_on_negative_strand: 638^639"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            634..647
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(640..647,641^642)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 641^642"
                     /note="cut_0_on_negative_strand: 645^646"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccat gtgctggttc aagttgtggt ctctcttgct ggtcggttca
       61 ctgctggtat ctggaacgcg gggcaagttg ccggacgccc ccgagtttga aaaggatctt
      121 ctcattcaga gactcaattg gatgctatgg gtgatcgatg aatgcttccg cgacctctgt
      181 taccgtaccg gcatctgcaa gggtattcta gagcccgctg ctatttttca tctgaaacta
      241 ccagccatca acgatactga tcactgcggg ttaataggat ttaatgagac tagctgcctt
      301 aaaaagctcg ccgatggctt ttttgaattc gaggtgttgt ttaagttttt aacgacggag
      361 tttggaaaat cagtgataaa cgtggacgtc atggagcttc tgacgaagac cttaggatgg
      421 gacatacagg aagagctcaa taagctgact aagacgcact acagtccacc caaatttgac
      481 cgcggtctat tagggaggct tcagggactt aagtattggg tgagacactt tgcttcgttt
      541 tatgttctga gtgcaatgga aaagtttgca ggtcaagcgg tgcgtgtttt ggactctatc
      601 ccagacgtga ctcctgacgt ccacgataag taactcgagg cggccgc
//