LOCUS pOME0005R 647 bp DNA linear UNA 14-Oct-2013 DEFINITION KSHV ORF K2; UNC154-5; vector pVR21 SOURCE KSHV ORGANISM KSHV KSHV ORF K2; UNC154-5; Synthetic Construct. REFERENCE 1 (bases 1 to 887) AUTHORS Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P., Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y., Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and Texier,J.R. TITLE Unpublished COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of the Orfeome project "R" in name = in replicon vector= pVR21 FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..633 /cds_type=ORF /note="Length: 615" /note="Found at strand: positive" /note="Start codon: atg" Site join(207..212,207^208) /site_type="restriction site" /note="Name: XbaI" /note="Pattern: tctaga" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 207^208" /note="cut_0_on_negative_strand: 211^212" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=XbaI Site join(325..330,325^326) /site_type="restriction site" /note="Name: EcoRI" /note="Pattern: gaattc" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 325^326" /note="cut_0_on_negative_strand: 329^330" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=EcoRI Site join(634..639,634^635) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 634^635" /note="cut_0_on_negative_strand: 638^639" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 634..647 /site_type="mutagenized site" /label="3' RE tag" Site join(640..647,641^642) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="Number_of_matches: 1" /note="cut_0_on_positive_strand: 641^642" /note="cut_0_on_negative_strand: 645^646" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccat gtgctggttc aagttgtggt ctctcttgct ggtcggttca 61 ctgctggtat ctggaacgcg gggcaagttg ccggacgccc ccgagtttga aaaggatctt 121 ctcattcaga gactcaattg gatgctatgg gtgatcgatg aatgcttccg cgacctctgt 181 taccgtaccg gcatctgcaa gggtattcta gagcccgctg ctatttttca tctgaaacta 241 ccagccatca acgatactga tcactgcggg ttaataggat ttaatgagac tagctgcctt 301 aaaaagctcg ccgatggctt ttttgaattc gaggtgttgt ttaagttttt aacgacggag 361 tttggaaaat cagtgataaa cgtggacgtc atggagcttc tgacgaagac cttaggatgg 421 gacatacagg aagagctcaa taagctgact aagacgcact acagtccacc caaatttgac 481 cgcggtctat tagggaggct tcagggactt aagtattggg tgagacactt tgcttcgttt 541 tatgttctga gtgcaatgga aaagtttgca ggtcaagcgg tgcgtgtttt ggactctatc 601 ccagacgtga ctcctgacgt ccacgataag taactcgagg cggccgc //