LOCUS pOME0012 482 bp DNA linear UNA 17-Oct-2013 DEFINITION KSHV K7 tagged; UNC154-12; vector pDEST47 SOURCE KSHV ORGANISM KSHV KSHV K7 tagged; UNC154-12; Synthetic Construct. REFERENCE 1 (bases 1 to 887) AUTHORS Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P., Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y., Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and Texier,J.R. TITLE Unpublished COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of Orfeome project FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..465 /cds_type=ORF /note="Length: 447" /note="Found at strand: positive" /note="Start codon: atg" Site 397..462 /site_type="mutagenized site" /label="3X FLAG tag" Site 463..468 /site_type="mutagenized site" /label="Double stop" Site join(469..474,469^470) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 469^470" /note="cut_0_on_negative_strand: 473^474" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 470..482 /site_type="mutagenized site" /label="3' RE tag" Site join(475..482,476^477) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 476^477" /note="cut_0_on_negative_strand: 480^481" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccat gggaacactg gagataaaag gggccagctt gagtcagttt 61 agcactggga ctgcccagtc accttggctg ccgcttcacc tatggatttt gtgctcgctg 121 cttgccttct tgccgcttct ggttttcatt ggtgccgccg attgtgggtt gattgcgtcg 181 cttttggcaa tatacccatc ctggctttcg gctaggtttt ccgtcctact tttcccacat 241 tggcctgaga gctgtagtac aaaaaacacc gcgcggtctg gagctctcca taagcccgca 301 gaacaaaagc tgcgatttgc ccaaaaacct tgccatggca actatacagt caccccttgc 361 gggttattgc attggattca atctccaggc cagttggact acaaagacca tgacggtgat 421 tataaagatc atgatatcga ttacaaggat gacgatgaca agtagtaact cgaggcggcc 481 gc //