LOCUS       pOME0012       482 bp    DNA     linear   UNA 17-Oct-2013
DEFINITION  KSHV K7 tagged; UNC154-12; vector pDEST47
SOURCE      KSHV
  ORGANISM  KSHV
            KSHV K7 tagged; UNC154-12; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of Orfeome project
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..465
                     /cds_type=ORF
                     /note="Length: 447"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            397..462
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            463..468
                     /site_type="mutagenized site"
                     /label="Double stop"
     Site            join(469..474,469^470)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 469^470"
                     /note="cut_0_on_negative_strand: 473^474"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            470..482
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(475..482,476^477)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 476^477"
                     /note="cut_0_on_negative_strand: 480^481"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccat gggaacactg gagataaaag gggccagctt gagtcagttt
       61 agcactggga ctgcccagtc accttggctg ccgcttcacc tatggatttt gtgctcgctg
      121 cttgccttct tgccgcttct ggttttcatt ggtgccgccg attgtgggtt gattgcgtcg
      181 cttttggcaa tatacccatc ctggctttcg gctaggtttt ccgtcctact tttcccacat
      241 tggcctgaga gctgtagtac aaaaaacacc gcgcggtctg gagctctcca taagcccgca
      301 gaacaaaagc tgcgatttgc ccaaaaacct tgccatggca actatacagt caccccttgc
      361 gggttattgc attggattca atctccaggc cagttggact acaaagacca tgacggtgat
      421 tataaagatc atgatatcga ttacaaggat gacgatgaca agtagtaact cgaggcggcc
      481 gc
//
