LOCUS pOME0014 629 bp DNA linear UNA 17-Oct-2013 DEFINITION KSHV ORF 16 tagged; UNC154-14; vector pDEST47 SOURCE KSHV ORGANISM KSHV KSHV ORF 16 tagged; UNC154-14; Synthetic Construct. REFERENCE 1 (bases 1 to 887) AUTHORS Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P., Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y., Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and Texier,J.R. TITLE Unpublished COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of Orfeome project FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..612 /cds_type=ORF /note="Length: 594" /note="Found at strand: positive" /note="Start codon: atg" Site 544..609 /site_type="mutagenized site" /label="3x FLAG tag" Site 610..615 /site_type="mutagenized site" /label="double stop" Site join(616..621,616^617) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 616^617" /note="cut_0_on_negative_strand: 620^621" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 616..629 /site_type="mutagenized site" /label="3' RE tag" Site join(622..629,623^624) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 623^624" /note="cut_0_on_negative_strand: 627^628" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccat ggacgaggac gttttgcctg gagaggtgtt ggccattgaa 61 gggatattca tggcctgtgg attaaacgaa cctgagtacc tgtaccatcc tttgctcagc 121 cctattaagc tatacatcac aggcttaatg cgagacaagg agtctttatt cgaggccatg 181 ttggctaatg tgagatttca cagcaccacc ggtataaacc agcttgggtt gagcatgctg 241 caggttagcg gcgatggaaa catgaactgg gggcgagccc tggctatact gacctttggc 301 agttttgtgg cccagaagtt atccaacgaa cctcacctgc gagactttgc tttggccgtt 361 ttacctgtat atgcgtatga agcaatcgga ccccagtggt ttcgcgctcg cggaggctgg 421 cgaggcctga aggcgtattg tacacaggtg cttaccagaa gaaggggacg gagaatgaca 481 gcgctattgg gaagcattgc attattggcc actatattgg cagcggtcgc gatgagcagg 541 agagactaca aagaccatga cggtgattat aaagatcatg atatcgatta caaggatgac 601 gatgacaagt agtaactcga ggcggccgc //