LOCUS       pOME0014       629 bp    DNA     linear   UNA 17-Oct-2013
DEFINITION  KSHV ORF 16 tagged; UNC154-14; vector pDEST47
SOURCE      KSHV
  ORGANISM  KSHV
            KSHV ORF 16 tagged; UNC154-14; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing
            through Genewiz
            pOME= plasmid of Orfeome project
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..612
                     /cds_type=ORF
                     /note="Length: 594"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            544..609
                     /site_type="mutagenized site"
                     /label="3x FLAG tag"
     Site            610..615
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(616..621,616^617)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 616^617"
                     /note="cut_0_on_negative_strand: 620^621"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            616..629
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(622..629,623^624)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 623^624"
                     /note="cut_0_on_negative_strand: 627^628"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccat ggacgaggac gttttgcctg gagaggtgtt ggccattgaa
       61 gggatattca tggcctgtgg attaaacgaa cctgagtacc tgtaccatcc tttgctcagc
      121 cctattaagc tatacatcac aggcttaatg cgagacaagg agtctttatt cgaggccatg
      181 ttggctaatg tgagatttca cagcaccacc ggtataaacc agcttgggtt gagcatgctg
      241 caggttagcg gcgatggaaa catgaactgg gggcgagccc tggctatact gacctttggc
      301 agttttgtgg cccagaagtt atccaacgaa cctcacctgc gagactttgc tttggccgtt
      361 ttacctgtat atgcgtatga agcaatcgga ccccagtggt ttcgcgctcg cggaggctgg
      421 cgaggcctga aggcgtattg tacacaggtg cttaccagaa gaaggggacg gagaatgaca
      481 gcgctattgg gaagcattgc attattggcc actatattgg cagcggtcgc gatgagcagg
      541 agagactaca aagaccatga cggtgattat aaagatcatg atatcgatta caaggatgac
      601 gatgacaagt agtaactcga ggcggccgc
//