LOCUS pOME0016 1319 bp DNA linear UNA 17-Oct-2013 DEFINITION KSHV ORF45 tagged; UNC154-16; vector pDEST47 SOURCE UNC154-16 (KSHV ORF45 tagged) COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of Orfeome project BamHI at 5' end for cloning FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: BamHI" /note="Pattern: ggatcc" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N4-methylcytosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=BamHI Site 1..6 /site_type="mutagenized site" /label="5' RE tag" Site 7..12 /site_type="mutagenized site" /label="Kozak sequence" CDS 13..1302 /cds_type=ORF /note="Length: 1290" /note="Found at strand: positive" /note="Start codon: atg" Site 1234..1299 /site_type="mutagenized site" /label="3X FLAG tag" Site 1300..1305 /site_type="mutagenized site" /label="double stop" Site join(1306..1311,1306^1307) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 1306^1307" /note="cut_0_on_negative_strand: 1310^1311" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 1306..1319 /site_type="mutagenized site" /label="3' RE tag" Site join(1312..1319,1313^1314) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 1313^1314" /note="cut_0_on_negative_strand: 1317^1318" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 ggatccgcca ccatggcgat gtttgtgagg acctcgtcta gcacacacga tgaagagaga 61 atgcttccaa ttgaaggagc gcctcgcaga cgaccccccg tgaagttcat attcccacct 121 ccacctcttt catcacttcc aggatttggc aggccgcgcg gctatgctgg acccacggtg 181 atagatatgt ctgccccaga cgacgtcttc gccgaggaca cgccatcgcc gccagcaacc 241 cctctggatc tacagatatc cccggatcag tcgagcggcg aatctgaata tgacgaggat 301 gaggaagatg aagatgaaga agaaaatgac gatgttcagg aggaagacga gccagagggg 361 taccctgcag acttttttca acctttatct cacttgcgcc cgaggcctct ggccagacgg 421 gcccatacgc ccaaaccggt agcagtggta gcgggccgcg tgcgcagttc aacggacacg 481 gcggagtccg aggcgtccat gggatgggtt agtcaggatg acggattttc ccctgctggg 541 ctctcacctt cagacgacga gggggttgct atcctggaac cgatggcggc atacactggg 601 accggggcat acggactttc acctgcttcc agaaatagtg tacctggaac acaaagttca 661 ccatacagcg accctgatga agggccctcg tggcgccccc tgcgcgccgc acccaccgcg 721 atcgtcgacc tgacatcgga ctctgatagc gatgacagtt ccaactctcc ggacgtgaac 781 aatgaggccg cgtttaccga cgcgcgccat ttttcccacc agccaccctc gtccgaggag 841 gacggagaag accaagggga agtattgagt cagagaatcg ggctcatgga cgtgggccag 901 aagcgcaaaa ggcagtctac cgcctcctct ggtagcgagg atgtggtgcg ctgccagaga 961 caaccaaact taagccgcaa agcagtggcg tccgtgataa ttatatcctc ggggagtgac 1021 acagacgagg agccctcgtc cgccgtgagc gtgatcgtgt ctccgtcgag cacaaagggt 1081 cacctcccaa cccaatctcc cagtacttcc gcccactcga tttcatcagg aagcacaact 1141 accgcggggt ccaggtgcag cgacccaacc cgcatcctgg cctccacgcc acccctgtgt 1201 ggaaacggtg catataactg gccgtggctg gacgactaca aagaccatga cggtgattat 1261 aaagatcatg atatcgatta caaggatgac gatgacaagt agtaactcga ggcggccgc //