LOCUS pOME0018R 605 bp DNA linear UNA 17-Oct-2013 DEFINITION KSHV ORF 47 tagged; UNC154-18; vector pDEST47 SOURCE KSHV ORGANISM KSHV KSHV ORF 47 tagged; UNC154-18; Synthetic Construct. REFERENCE 1 (bases 1 to 887) AUTHORS Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P., Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y., Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and Texier,J.R. TITLE Unpublished COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of Orfeome project FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..588 /cds_type=ORF /note="Length: 570" /note="Found at strand: positive" /note="Start codon: atg" Site 520..585 /site_type="mutagenized site" /label="3X FLAG tag" Site 586..591 /site_type="mutagenized site" /label="double stop" Site join(592..597,592^593) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 592^593" /note="cut_0_on_negative_strand: 596^597" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 592..605 /site_type="mutagenized site" /label="3' RE tag" Site join(598..605,599^600) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 599^600" /note="cut_0_on_negative_strand: 603^604" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccat ggggatcttt gcgctatttg ccgtcctgtg gaccacccta 61 ttggtcacat ctcacgcata cgtcgcctta ccatgttgcg caattcaggc atcggcagcc 121 tctaccctgc cgttgttctt tgcggtccac tctatccact tcgccgatcc gaatcactgc 181 aacggggtct gtatagccaa gctgcgaagc aaaacaggcg acattaccgt ggaaacatgc 241 gtgaatgggt ttaatctgag gtcattttta gtcgcggtcg ttcgaagatt ggggtcctgg 301 gcgtcgcagg aaaacctgag gttgttgtgg tatttacaac gaagtttgac ggcctatact 361 gtaggtttta acgcgaccac tgcagatagc tctattcaca acgtaaacat aattataata 421 agcgtgggaa aggccatgaa ccggacaggt tctgttagcg gaagtcagac tcgggctaaa 481 agcagcagcc ggagagcgca cgcaggtcaa aagggaaaag actacaaaga ccatgacggt 541 gattataaag atcatgatat cgattacaag gatgacgatg acaagtagta actcgaggcg 601 gccgc //