LOCUS pOME0020 434 bp DNA linear UNA 17-Oct-2013 DEFINITION KSHV ORF 53 tagged; UNC154-20; vector pDEST47 SOURCE KSHV ORGANISM KSHV KSHV ORF 53 tagged; UNC154-20; Synthetic Construct. REFERENCE 1 (bases 1 to 887) AUTHORS Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P., Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y., Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and Texier,J.R. TITLE Unpublished COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of Orfeome project Did not express FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..417 /cds_type=ORF /note="Length: 399" /note="Found at strand: positive" /note="Start codon: atg" Site 349..414 /site_type="mutagenized site" /label="3x FLAG tag" Site 415..420 /site_type="mutagenized site" /label="double stop" Site join(421..426,421^422) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 421^422" /note="cut_0_on_negative_strand: 425^426" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 421..434 /site_type="mutagenized site" /label="3' RE tag" Site join(427..434,428^429) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 428^429" /note="cut_0_on_negative_strand: 432^433" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccat gacagcgtcc acggtggccc tggctttatt tgtggcctcg 61 atactaggtc actgctgggt tacggccaac tcgacaggtg tcgcatcatc tacagagcgt 121 tctagtccca gtacggctgg gctcagcgcc aggccttcgc ccggtcccac atcagtcact 181 accccaggat tctatgacgt tgcttgttcc gcggatagtt ttagcccctc cctttctagc 241 ttttctagcg tctgggcctt gattaacgcg ttactagtcg tcgtggccac atttttctat 301 ctggtatatc tttgtttttt caagtttgtg gacgaggtgg tccacgcaga ctacaaagac 361 catgacggtg attataaaga tcatgatatc gattacaagg atgacgatga caagtagtaa 421 ctcgaggcgg ccgc //