LOCUS       pOME0020       434 bp    DNA     linear   UNA 17-Oct-2013
DEFINITION  KSHV ORF 53 tagged; UNC154-20; vector pDEST47
SOURCE      KSHV
  ORGANISM  KSHV
            KSHV ORF 53 tagged; UNC154-20; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of Orfeome project
            Did not express
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..417
                     /cds_type=ORF
                     /note="Length: 399"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            349..414
                     /site_type="mutagenized site"
                     /label="3x FLAG tag"
     Site            415..420
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(421..426,421^422)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 421^422"
                     /note="cut_0_on_negative_strand: 425^426"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            421..434
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(427..434,428^429)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 428^429"
                     /note="cut_0_on_negative_strand: 432^433"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccat gacagcgtcc acggtggccc tggctttatt tgtggcctcg
       61 atactaggtc actgctgggt tacggccaac tcgacaggtg tcgcatcatc tacagagcgt
      121 tctagtccca gtacggctgg gctcagcgcc aggccttcgc ccggtcccac atcagtcact
      181 accccaggat tctatgacgt tgcttgttcc gcggatagtt ttagcccctc cctttctagc
      241 ttttctagcg tctgggcctt gattaacgcg ttactagtcg tcgtggccac atttttctat
      301 ctggtatatc tttgtttttt caagtttgtg gacgaggtgg tccacgcaga ctacaaagac
      361 catgacggtg attataaaga tcatgatatc gattacaagg atgacgatga caagtagtaa
      421 ctcgaggcgg ccgc
//