LOCUS pOME0022 377 bp DNA linear UNA 18-Oct-2013 DEFINITION HKU4 ORF 3 tagged; vector pDEST47 SOURCE HKU4 ORGANISM HKU4 HKU4 NS3a ORF 3 tagged; UNC163-2; Synthetic Construct. REFERENCE 1 (bases 1 to 887) AUTHORS Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P., Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y., Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and Texier,J.R. TITLE Unpublished COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of the Orfeome project Did not express FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..363 /cds_type=ORF /note="Length: 276" /note="Found at strand: positive" /note="Start codon: ATG" Site 292..357 /site_type="mutagenized site" /label="3x FLAG tag" Site 358..363 /site_type="mutagenized site" /label="Double stop" Site join(364..369,364^365) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 364^365" /note="cut_0_on_negative_strand: 368^369" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 364..377 /site_type="mutagenized site" /label="3' RE tag" Site join(370..377,371^372) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 371^372" /note="cut_0_on_negative_strand: 375^376" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccAT GGTTTCTTTT AATGCTACTG CTATTCTTCT TTTATTACTT 61 GCTAATGCTT TTTCTAAACC TTTATATGTC CCTGAGCATT GTGGTGGCAT GTCTGGCACT 121 CTCTTTCAGG CTTGTATTAG ACAAACTATG GTTGATACAA CCGGTATGTA CACCAATTCA 181 GCTATGTCAC ATGATGGTGT TACAATACCA TTTGATAGAG ATGGCATTGT ACATGAAGAT 241 CATTACACTG AAACAAACCC TACACCACTT TTTGATGCTG GGTTTTCCGT Agactacaaa 301 gaccatgacg gtgattataa agatcatgat atcgattaca aggatgacga tgacaagtag 361 taactcgagg cggccgc //