LOCUS       pOME0024       461 bp    DNA     linear   UNA 18-Oct-2013
DEFINITION  HKU4 ORF 4a tagged; vector pDEST47
SOURCE      HKU4
  ORGANISM  HKU4
            HKU4 ORF 4a tagged; UNC163-20; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of Orfeome project
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE site"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..444
                     /cds_type=ORF
                     /note="Length: 426"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            376..441
                     /site_type="mutagenized site"
                     /label="3x FLAG tag"
     Site            442..447
                     /site_type="mutagenized site"
                     /label="Double stop"
     Site            join(448..453,448^449)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 448^449"
                     /note="cut_0_on_negative_strand: 452^453"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            448..461
                     /site_type="mutagenized site"
                     /label="3' RE site"
     Site            join(454..461,455^456)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 455^456"
                     /note="cut_0_on_negative_strand: 459^460"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GGACTACGTC TCTTTGCTTA ACCAATTTTG GCAGAAGCAA
       61 ATTAAGTCTT ATAAAGAGAC TCCTAGTCAG TATCATTACC TGTATCCACC CAGGTTTTTC
      121 TATAAACCTG TTTTGGGTAA TTTACAGCAC CCTACCAAGT GGTGTTGTAC TATTAAATTT
      181 TATGAGTATA GTGCTCAGGC TACTGAGTGT ACTAAAGCAT CAGCAAAACA AGATGCAGCT
      241 AGACTTATCT GTGAACAGTT ACAAGCTGCT GGACTACTCA ATGGAATGGA GCTCCGATTC
      301 CGTAGTTCTG CCTCCGACAT CTTCGGACAA AACCGTTATG ATGCCAGCAA AAGCTACTTC
      361 TTCTCGAAAA CGGCAgacta caaagaccat gacggtgatt ataaagatca tgatatcgat
      421 tacaaggatg acgatgacaa gtagtaactc gaggcggccg c
//
