LOCUS       pOME0026       953 bp    DNA     linear   UNA 18-Oct-2013
DEFINITION  HKU4 ORF 4b tagged; vector pDEST47
SOURCE      HKU4
  ORGANISM  HKU4
            HKU4 NS3c ORF 4b tagged; UNC 163-4; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of Orfeome project
            Only NotI site at 3' end due to internal XhoI
            Did not express
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..942
                     /cds_type=ORF
                     /note="Length: 924"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            874..939
                     /site_type="mutagenized site"
                     /label="3x FLAG tag"
     Site            940..945
                     /site_type="mutagenized site"
                     /label="Double stop"
     Site            join(946..953,947^948)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 947^948"
                     /note="cut_0_on_negative_strand: 951^952"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
     Site            946..953
                     /site_type="mutagenized site"
                     /label="3' RE tag"
ORIGIN
        1 gtcgacggta ccgccaccAT GAGTATAGTG CTCAGGCTAC TGAGTGTACT AAAGCATCAG
       61 CAAAACAAGA TGCAGCTAGA CTTATCTGTG AACAGTTACA AGCTGCTGGA CTACTCAATG
      121 GAATGGAGCT CCGATTCCGT AGTTCTGCCT CCGACATCTT CGGACAAAAC CGTTATGATG
      181 CCAGCAAAAG CTACTTCTTC TCGAAAACGG CATAGAAAAC CTAAATTGCA ATATGCCAAG
      241 AGAAGGTTTT CCCCTGTTAA TCCAAATGAC TTAGTGCTAG TTCAGCAAGA ACCTACTCAC
      301 TGTGTTCGTT TGGTTTTTCC TTTAAGTAAG CGCTGGATAC ATTTTGATGG ACTCGTCTAT
      361 TCTTTAGCGC GTCTTAATGT GTCTATGACT GTTGATTATC ATGTTACCCT AGCTCTCATC
      421 TATGCTCCTG AGGCTGGTGA ATGCTTTGGT AACTTTTTAC ATCTTTGTCC ATTGCTTAAA
      481 GATTGCTTGT TGGAGTTTAA AAAGCTCTGT GTTCTTGGCA AAACACTTAC CATTTTGGCA
      541 AGTGAATGGC CATTTTTTAC AGATGTTAAA AAGAATAAAG ACAATTTAAC TGTTCCTAAA
      601 GCTGTAGAGT GGCTAAAGGA ACACGGCTAT GAGATATATA ATTCTCAATT GCCTTTACAT
      661 ATGTCTTTAG CAAAATTACA TGACTTACCA CAAGCACAGT TTGCTGAAGC GGCTGGTTTA
      721 TGTCATTATT TTGATCCTCG AGAGTTTGCT TTACCTTGCG CTCTTGAAGT AGTTAAGATA
      781 GGTGGTGGTA AAGTTAATGG TAGATCCATT CCACTTGCCC GCTTCCCAAT TAATAATGAG
      841 TTTAAATTCA TTCCTTATCT TTATCAATGT GTTgactaca aagaccatga cggtgattat
      901 aaagatcatg atatcgatta caaggatgac gatgacaagt agtaagcggc cgc
//