LOCUS pOME0027R 716 bp DNA linear UNA 18-Oct-2013 DEFINITION HKU4 ORF 5; vector pVR21 SOURCE HKU4 ORGANISM HKU4 HKU4 NS3D ORF 5; UNC163-5; Synthetic Construct. REFERENCE 1 (bases 1 to 887) AUTHORS Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P., Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y., Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and Texier,J.R. TITLE Unpublished COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of the Orfeome plasmid "R" in name= in replicon plasmi= pVR21 FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..702 /cds_type=ORF /note="Length: 684" /note="Found at strand: positive" /note="Start codon: ATG" Site join(703..708,703^704) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 703^704" /note="cut_0_on_negative_strand: 707^708" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 703..716 /site_type="mutagenized site" /label="3' RE tag" Site join(709..716,710^711) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 710^711" /note="cut_0_on_negative_strand: 714^715" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccAT GGCTTTTTCA GCTTCTTTAT TTCGGACTAA AACAGTCCAT 61 ACTGAAGATG CATTTTGCCC TCGTTCGGCT ATTCAGGCTG AACAGCCTCC TAATATCATA 121 GACTGCATAC CAGTTGCAGG CTATGAAGCT GCATTAATCA CTAATGCACT TTTCCTATTA 181 GTATTATTTG TCTTTAACCC ACTTACCTGT AAGGGCAATT GGATTAAAGC TATTTTGTTT 241 TACAGCTTAT TGCTGTATAA TATGATATTA GCCATATTTT TGGTTGTTGA TACACAACAT 301 TTTGTTAGTG CTTTGTTGCT AGCTTATGTT GTTACTTTTC TAGTCTTGTG GACTGCTGAC 361 CGAATACGTC TCAGTTGTGC AGTAGGAAGT GTATTACCCT TTGTCGATAT GCGGTCTTCC 421 TATATCCGTG TTGACAACGG TAATTCCAGT GTTGTAGTTC CAATGAACCA CACAAAGCAC 481 TGGTTTATAA GAAATTTTGA GCAATCTTGT CATTGTGAAA ATTGTTTTTA TATTCACTCT 541 TCATCTTATG TTGAGTGTAC TTTTATTAGT CGCCTAAAGA AATCCATACT TGTTTCAGTT 601 TGTGATTTTT CTTTAGGTGG TAATGTTTCT ACTGTTTTTG TCCCTTCTAG TGATAAAACA 661 GTACCCCTTC ACATAATCGC ACCTAGCAAG CTTTATGTCT AActcgaggc ggccgc //