LOCUS       pOME0036       767 bp    DNA     linear   UNA 22-Oct-2013
DEFINITION  HKU5 ORF 5 tagged; vector pDEST47
SOURCE      HKU5
  ORGANISM  HKU5
            HKU5 ORF 5 tagged; UNC163-8; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of Orfeome project
            Did not express
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..756
                     /cds_type=ORF
                     /note="Length: 738"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            688..753
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            754..759
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(760..767,761^762)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 761^762"
                     /note="cut_0_on_negative_strand: 765^766"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
     Site            760..767
                     /site_type="mutagenized site"
                     /label="3' RE tag"
ORIGIN
        1 gtcgacggta ccgccaccAT GGCTTTTTCG CCATCCCTGT TTCAGCCACT TGTCATCCAA
       61 AAGGAGACAC ACGGTGGTGA ACCTAGCAGC CCTAATCATG TCATCGCATG CATACCTCTT
      121 ACAGGCTATG TTGCCGCTCT TGTGGTTAAT GCATGCTTTT ACCCTCTACT ATTCTGCTTA
      181 CCTTATAGCA GTTGTAGGGC TAGTGTATGT AAGACCTTGG TACTCTATGT GCTGATGCTT
      241 TACAATTTCA TTTTGTCGTG TATCCTAGTT GAGGACACAC AACAACCTGT TGGCATATGC
      301 CTTATGGTAT ATTGCATAAT ATTAATGGCG ATCTGGACTA TCGACAGAGT TCGATTTTGT
      361 CTGCTGATCA GATCATTACG TCCACTTATT GACATGAGGT CAAATTTCAT CCGTGTCAAT
      421 ACTGTTGCAG GCGGGGTTGT TATACCTGTT AACTATAGCA AACCCTGGTT TGTCAAAAAT
      481 TTTAACCAGC GTTGTCGCTG TACAAATTGT TTCTTTGCGC ACTCAGCAAC CTATCTCGAG
      541 TGCACATTTA TAAGCCGTTT CTCTAAAACC ACTCTTGTTT CCATTAGTGA CTTTCAGCTT
      601 AACGGTTCTC ATTCAACTGT TTTCGTGCCT TTCAATAGCC GCGATTCAGT ACCTCTTCAC
      661 ATAATCGCAC CCAGCGTGCT TACAGTTgac tacaaagacc atgacggtga ttataaagat
      721 catgatatcg attacaagga tgacgatgac aagtagtaag cggccgc
//
