LOCUS pOME0040 566 bp DNA linear UNA 23-Oct-2013 DEFINITION SARS ORF 3b nt 25689-26153 tagged; vector pDEST47 SOURCE SARS ORGANISM SARS SARS ORF 3b tagged; UNC160-2; Synthetic Construct. REFERENCE 1 (bases 1 to 887) AUTHORS Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P., Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y., Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and Texier,J.R. TITLE Unpublished COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of Orfeome project FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..>566 /cds_type=ORF /note="Length: 465" /note="Found at strand: positive" /note="Start codon: ATG" Site 481..546 /site_type="mutagenized site" /label="3X FLAG tag" Site 547..552 /site_type="mutagenized site" /label="double stop" Site join(553..558,553^554) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 553^554" /note="cut_0_on_negative_strand: 557^558" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 553..566 /site_type="mutagenized site" /label="3' RE tag" Site join(559..566,560^561) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 560^561" /note="cut_0_on_negative_strand: 564^565" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccAT GATGCCAACT ACTTTGTTTG CTGGCACACA CATAACTATG 61 ACTACTGTAT ACCATATAAC AGTGTCACAG ATACAATTGT CGTTACTGAA GGTGACGGCA 121 TTTCAACACC AAAACTCAAA GAAGACTACC AAATTGGTGG TTATTCTGAG GATAGGCACT 181 CAGGTGTTAA AGACTATGTC GTTGTACATG GCTATTTCAC CGAAGTTTAC TACCAGCTTG 241 AGTCTACACA AATTACTACA GACACTGGTA TTGAAAATGC TACATTCTTC ATCTTTAACA 301 AGCTTGTTAA AGACCCACCG AATGTGCAAA TACACACAAT CGACGGCTCT TCAGGAGTTG 361 CTAATCCAGC AATGGATCCA ATTTATGATG AGCCGACGAC GACTACTAGC GTGCCTTTGT 421 AAGCACAAGA AAGTGAGTAC GAACTTATGT ACTCATTCGT TTCGGAAGAA ACAGGTACGT 481 gactacaaag accatgacgg tgattataaa gatcatgata tcgattacaa ggatgacgat 541 gacaagtagt aactcgaggc ggccgc //