LOCUS       pOME0040CO       566 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  Human codon optimized SARS ORF 3b nt 25689-26153 tagged; vector pDEST47
SOURCE      SARS
  ORGANISM  SARS
            SARS ORF 3b tagged; UNC160-2; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic 
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of Orfeome project
            "CO" in name= human codon optimized
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..549
                     /cds_type=ORF
                     /note="Length: 531"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            481..546
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            547..552
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(553..558,553^554)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 553^554"
                     /note="cut_0_on_negative_strand: 557^558"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            553..566
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(559..566,560^561)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 560^561"
                     /note="cut_0_on_negative_strand: 564^565"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GATGCCTACC ACCCTTTTCG CTGGCACACA TATCACTATG
       61 ACCACCGTGT ACCATATCAC AGTGTCCCAG ATCCAGCTCT CCCTTTTGAA GGTGACCGCA
      121 TTCCAGCACC AAAACAGCAA GAAGACCACT AAGCTCGTGG TCATCCTGAG AATTGGGACC
      181 CAGGTGTTGA AGACCATGAG CTTGTACATG GCCATTAGCC CCAAGTTCAC AACTAGCCTG
      241 TCCCTGCACA AGCTGCTCCA GACATTGGTC CTGAAGATGC TGCACTCTTC ATCCCTGACA
      301 TCTCTGCTGA AGACACACAG GATGTGCAAG TATACACAGT CCACAGCCCT CCAGGAGCTT
      361 CTGATTCAGC AGTGGATTCA GTTTATGATG AGCAGGCGGA GGCTCCTCGC CTGCCTGTGC
      421 AAACACAAAA AGGTGAGCAC TAATCTGTGT ACTCACAGTT TTCGGAAGAA ACAGGTCCGC
      481 gactacaaag accatgacgg tgattataaa gatcatgata tcgattacaa ggatgacgat
      541 gacaagtagt aactcgaggc ggccgc
//