LOCUS       pOME0044       470 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  SARS ORF 7a nt 27273-27641 tagged; vector pDEST47
SOURCE      SARS
  ORGANISM  SARS
            SARS ORF 7a tagged; UNC160-4; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verfied via Sanger sequencing through Genewiz
            pOME= plasmid of Orfeome project
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..453
                     /cds_type=ORF
                     /note="Length: 435"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            385..450
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            451..456
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(457..462,457^458)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 457^458"
                     /note="cut_0_on_negative_strand: 461^462"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            457..470
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(463..470,464^465)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 464^465"
                     /note="cut_0_on_negative_strand: 468^469"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GAAAATTATT CTCTTCCTGA CATTGATTGT ATTTACATCT
       61 TGCGAGCTAT ATCACTATCA GGAGTGTGTT AGAGGTACGA CTGTACTACT AAAAGAACCT
      121 TGCCCATCAG GAACATACGA GGGCAATTCA CCATTTCACC CTCTTGCTGA CAATAAATTT
      181 GCACTAACTT GCACTAGCAC ACACTTTGCT TTTGCTTGTG CTGACGGTAC TCGACATACC
      241 TATCAGCTGC GTGCAAGATC AGTTTCACCA AAACTTTTCA TCAGACAAGA GGAGGTTCAA
      301 CAAGAGCTCT ACTCGCCACT TTTTCTCATT GTTGCTGCTC TAGTATTTTT AATACTTTGC
      361 TTCACCATTA AGAGAAAGAC AGAAgactac aaagaccatg acggtgatta taaagatcat
      421 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc
//