LOCUS pOME0046 230 bp DNA linear UNA 23-Oct-2013 DEFINITION SARS ORF 7b nt 27638-27772 tagged; vector pDEST47 SOURCE SARS ORGANISM SARS SARS ORF 7b tagged; UNC 160-6; Synthetic Construct. REFERENCE 1 (bases 1 to 887) AUTHORS Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P., Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y., Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and Texier,J.R. TITLE Unpublished COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of Orfeome project SalI site created by addition of FLAG tag so 5' site only has KpnI FEATURES Location/Qualifiers Site join(1..6,5^6) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 5^6" /note="cut_0_on_negative_strand: 1^2" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 1..6 /site_type="mutagenized site" /label="5' RE tag" Site 7..12 /site_type="mutagenized site" /label="Kozak sequence" CDS 13..213 /cds_type=ORF /note="Length: 201" /note="Found at strand: positive" /note="Start codon: ATG" Site 145..210 /site_type="mutagenized site" /label="3X FLAG tag" Site 211..216 /site_type="mutagenized site" /label="double stop" Site join(217..222,217^218) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 217^218" /note="cut_0_on_negative_strand: 221^222" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 217..230 /site_type="mutagenized site" /label="3' RE tag" Site join(223..230,224^225) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 224^225" /note="cut_0_on_negative_strand: 228^229" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 ggtaccgcca ccATGAATGA GCTCACTTTA ATTGACTTCT ATTTGTGCTT TTTAGCCTTT 61 CTGCTATTCC TTGTTTTAAT AATGCTTATT ATATTTTGGT TTTCACTCGA AATCCAGGAT 121 CTAGAAGAAC CTTGTACCAA AGTCgactac aaagaccatg acggtgatta taaagatcat 181 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc //