LOCUS       pOME0046       230 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  SARS ORF 7b nt 27638-27772 tagged; vector pDEST47
SOURCE      SARS
  ORGANISM  SARS
            SARS ORF 7b tagged; UNC 160-6; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of Orfeome project
            SalI site created by addition of FLAG tag so 5' site only has KpnI
FEATURES             Location/Qualifiers
     Site            join(1..6,5^6)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 5^6"
                     /note="cut_0_on_negative_strand: 1^2"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             13..213
                     /cds_type=ORF
                     /note="Length: 201"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            145..210
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            211..216
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(217..222,217^218)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 217^218"
                     /note="cut_0_on_negative_strand: 221^222"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            217..230
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(223..230,224^225)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 224^225"
                     /note="cut_0_on_negative_strand: 228^229"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 ggtaccgcca ccATGAATGA GCTCACTTTA ATTGACTTCT ATTTGTGCTT TTTAGCCTTT
       61 CTGCTATTCC TTGTTTTAAT AATGCTTATT ATATTTTGGT TTTCACTCGA AATCCAGGAT
      121 CTAGAAGAAC CTTGTACCAA AGTCgactac aaagaccatg acggtgatta taaagatcat
      181 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc
//
