LOCUS       pOME0048       221 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  SARS ORF 8a nt27779-27898 tagged; vector pDEST47
SOURCE      SARS
  ORGANISM  SARS
            SARS ORF 8a tagged; UNC 160-8; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of Orfeome project 
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..>221
                     /cds_type=ORF
                     /note="Length: 120"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            136..201
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            202..207
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(208..213,208^209)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 208^209"
                     /note="cut_0_on_negative_strand: 212^213"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            208..221
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(214..221,215^216)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 215^216"
                     /note="cut_0_on_negative_strand: 219^220"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GAAACTTCTC ATTGTTTTGA CTTGTATTTC TCTATGCAGT
       61 TGCATATGCA CTGTAGTACA GCGCTGTGCA TCTAATAAAC CTCATGTGCT TGAAGATCCT
      121 TGTAAGGTAC AACACgacta caaagaccat gacggtgatt ataaagatca tgatatcgat
      181 tacaaggatg acgatgacaa gtagtaactc gaggcggccg c
//
