LOCUS       pOME0050       350 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  SARS ORF 8b nt 27864-28118 tagged; vector pDEST47
SOURCE      SARS
  ORGANISM  SARS
            SARS ORF 8b tagged; UNC160-10; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz 
            pOME= plasmid of Orfeome project
            Internal KpnI site; only SalI at 5' end
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             13..333
                     /cds_type=ORF
                     /note="Length: 321"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            265..330
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            331..336
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(337..342,337^338)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 337^338"
                     /note="cut_0_on_negative_strand: 341^342"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            337..350
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(343..350,344^345)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 344^345"
                     /note="cut_0_on_negative_strand: 348^349"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacgcca ccATGTGCTT GAAGATCCTT GTAAGGTACA ACACTAGGGG TAATACTTAT
       61 AGCACTGCTT GGCTTTGTGC TCTAGGAAAG GTTTTACCTT TTCATAGATG GCACACTATG
      121 GTTCAAACAT GCACACCTAA TGTTACTATC AACTGTCAAG ATCCAGCTGG TGGTGCGCTT
      181 ATAGCTAGGT GTTGGTACCT TCATGAAGGT CACCAAACTG CTGCATTTAG AGACGTACTT
      241 GTTGTTTTAA ATAAACGAAC AAATgactac aaagaccatg acggtgatta taaagatcat
      301 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc
//