LOCUS       pOME0051N       394 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  SARS ORF 9b nt 28130-28426 with 5' 3x FLAG tag; vector pDEST47
SOURCE      SARS
  ORGANISM  SARS
            SARS ORF 9b; UNC160-11; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of Orfeome project
            "N" in name= FLAG tag has been moved to the N terminus
            Internal XhoI site so only NotI site at 3' end
FEATURES             Location/Qualifiers
     Site            1..17
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(6..11,6^7)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 6^7"
                     /note="cut_0_on_negative_strand: 10^11"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            join(12..17,16^17)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 16^17"
                     /note="cut_0_on_negative_strand: 12^13"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            18..23
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             24..386
                     /cds_type=ORF
                     /note="Length: 363"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            27..92
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            join(387..394,388^389)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 388^389"
                     /note="cut_0_on_negative_strand: 392^393"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
     Site            387..394
                     /site_type="mutagenized site"
                     /label="3' RE tag"
ORIGIN
        1 TCGCCGTCGA CGGTACCGCC ACCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC
       61 ATGATATCGA TTACAAGGAT GACGATGACA AGGACCCCAA TCAAACCAAC GTAGTGCCCC
      121 CCGCATTACA TTTGGTGGAC CCACAGATTC AACTGACAAT AACCAGAATG GAGGACGCAA
      181 TGGGGCAAGG CCAAAACAGC GCCGACCCCA AGGTTTACCC AATAATACTG CGTCTTGGTT
      241 CACAGCTCTC ACTCAGCATG GCAAGGAGGA ACTTAGATTC CCTCGAGGCC AGGGCGTTCC
      301 AATCAACACC AATAGTGGTC CAGATGACCA AATTGGCTAC TACCGAAGAG CTACCCGACG
      361 AGTTCGTGGT GGTGACGGCA AAATGAgcgg ccgc
//