LOCUS       pOME0052       392 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  SARS ORF 9b nt 28130-28426 tagged; vector pDEST47
SOURCE      SARS
  ORGANISM  SARS
            SARS ORF 9b tagged; UNC160-12; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of Orfeome project
            Internal XhoI site so only NotI site at 3' end
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..381
                     /cds_type=ORF
                     /note="Length: 363"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            313..378
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            379..384
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(385..392,386^387)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 386^387"
                     /note="cut_0_on_negative_strand: 390^391"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
     Site            385..392
                     /site_type="mutagenized site"
                     /label="3' RE tag"
ORIGIN
        1 gtcgacggta ccgccaccAT GGACCCCAAT CAAACCAACG TAGTGCCCCC CGCATTACAT
       61 TTGGTGGACC CACAGATTCA ACTGACAATA ACCAGAATGG AGGACGCAAT GGGGCAAGGC
      121 CAAAACAGCG CCGACCCCAA GGTTTACCCA ATAATACTGC GTCTTGGTTC ACAGCTCTCA
      181 CTCAGCATGG CAAGGAGGAA CTTAGATTCC CTCGAGGCCA GGGCGTTCCA ATCAACACCA
      241 ATAGTGGTCC AGATGACCAA ATTGGCTACT ACCGAAGAGC TACCCGACGA GTTCGTGGTG
      301 GTGACGGCAA AAgactacaa agaccatgac ggtgattata aagatcatga tatcgattac
      361 aaggatgacg atgacaagta gtaagcggcc gc
//