LOCUS pOME0056 221 bp DNA linear UNA 23-Oct-2013 DEFINITION HKU3 ORF 3b nt 25633-25762 tagged; vector pDEST47 SOURCE HKU3 ORGANISM HKU3 HKU3 ORF 3b tagged; UNC160-14; Synthetic Construct. REFERENCE 1 (bases 1 to 887) AUTHORS Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P., Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y., Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and Texier,J.R. TITLE Unpublished COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of Orfeome project Plasmid received from RG tagged FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..204 /cds_type=ORF /note="Length: 186" /note="Found at strand: positive" /note="Start codon: ATG" Site 136..201 /site_type="mutagenized site" /label="3X FLAG tag" Site 202..207 /site_type="mutagenized site" /label="double stop" Site join(208..213,208^209) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 208^209" /note="cut_0_on_negative_strand: 212^213" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 208..221 /site_type="mutagenized site" /label="3' RE tag" Site join(214..221,215^216) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 215^216" /note="cut_0_on_negative_strand: 219^220" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccAT GATGCTAACT ATTTTGTATG TTGGCATACT AATAACTATG 61 ACTACTGTAT ACCATACAAC AGTGTCACAG ATACAGTTGT CATCACCTCA GGTGATGGAA 121 CAAATCAGCC AAAACgacta caaagaccat gacggtgatt ataaagatca tgatatcgat 181 tacaaggatg acgatgacaa gtagtaactc gaggcggccg c //