LOCUS       pOME0059R       401 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  HKU3 ORF 7a nt 27217-27585; vector pVR21
SOURCE      HKU3
  ORGANISM  HKU3
            HKU3 ORF 7a; UNC160-15; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            "R" in name= in replicon vector= pVR21
            Plamsid received from RG
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..387
                     /cds_type=ORF
                     /note="Length: 369"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            join(388..393,388^389)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 388^389"
                     /note="cut_0_on_negative_strand: 392^393"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            388..401
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(394..401,395^396)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 395^396"
                     /note="cut_0_on_negative_strand: 399^400"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GAAAATTATT CTCTTCTTGA CATTGATAGC GCTTGCAACT
       61 TGTGAGTTAT ATCATTATCA GGAGTGTGTT AGGGGTACGA CTGTACTACT AAAAGAACCT
      121 TGCCCATCAG GAACATATGA GGGCAATTCA CCATTTCACC CTCTTGCTGA TAATAAATTT
      181 GCACTAACTT GCTCTAGCAC ACATTTTGCT TTTGCTTGTG CTGACGGTAC TAGACACACC
      241 TATCAGCTTC GTGCAAGATC AGTTTCACCA AAACTTTTCA TCAGACAAGA GGAAGTTTAT
      301 CAAGAGCTCT ACTCACCACT TTTCCTCATT GTTGCTGCAT TAGTATTTAT AATACTTTGC
      361 TTCACCATTA AGAGAAAGAC AGAATGActc gaggcggccg c
//