LOCUS       pOME0060       470 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  HKU3 ORF 7a nt 27217-27585; vector pDEST47 
SOURCE      HKU3
  ORGANISM  HKU3
            HKU3 ORF 7a tagged; UNC160-16; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project 
            Plasmid received from RG tagged
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..453
                     /cds_type=ORF
                     /note="Length: 435"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            386..450
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            451..456
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(457..462,457^458)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 457^458"
                     /note="cut_0_on_negative_strand: 461^462"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            457..470
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(463..470,464^465)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 464^465"
                     /note="cut_0_on_negative_strand: 468^469"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GAAAATTATT CTCTTCTTGA CATTGATAGC GCTTGCAACT
       61 TGTGAGTTAT ATCATTATCA GGAGTGTGTT AGGGGTACGA CTGTACTACT AAAAGAACCT
      121 TGCCCATCAG GAACATATGA GGGCAATTCA CCATTTCACC CTCTTGCTGA TAATAAATTT
      181 GCACTAACTT GCTCTAGCAC ACATTTTGCT TTTGCTTGTG CTGACGGTAC TAGACACACC
      241 TATCAGCTTC GTGCAAGATC AGTTTCACCA AAACTTTTCA TCAGACAAGA GGAAGTTTAT
      301 CAAGAGCTCT ACTCACCACT TTTCCTCATT GTTGCTGCAT TAGTATTTAT AATACTTTGC
      361 TTCACCATTA AGAGAAAGAC AGAAgactac aaagaccatg acggtgatta taaagatcat
      421 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc
//