LOCUS       pOME0063N       469 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  HKU3 ORF 8a nt 27723-28088 tagged; vector pDEST47
SOURCE      HKU3
  ORGANISM  HKU3
            HKU3 ORF 8a tagged; UNC160-19; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            "N" in name = N terminus 3x FLAG tag 
            Plasmid received from RG
FEATURES             Location/Qualifiers
     Site            1..17
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(6..11,6^7)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 6^7"
                     /note="cut_0_on_negative_strand: 10^11"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            join(12..17,16^17)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 16^17"
                     /note="cut_0_on_negative_strand: 12^13"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            18..23
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             24..455
                     /cds_type=ORF
                     /note="Length: 432"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            27..92
                     /site_type="mutagenized site"
                     /label="3x FLAG tag"
     Site            join(456..461,456^457)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 456^457"
                     /note="cut_0_on_negative_strand: 460^461"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            456..469
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(462..469,463^464)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 463^464"
                     /note="cut_0_on_negative_strand: 467^468"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 TCGCCGTCGA CGGTACCGCC ACCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC
       61 ATGATATCGA TTACAAGGAT GACGATGACA AGAAACTTCT CATTGTTTTT GGACTCTTAG
      121 CATCAGTGTA CTGCTTCCAC AGAGAATGCA GCATACAAGA GTGTTGTGAA AATCAACCCT
      181 ACCAAATTGA AGACCCATGT CCAATACATT ACTATTCGGA CTGGTTTATA AAAATTGGAT
      241 CTAGAAAATC GGCTCGCCTC GTACAATTGT GCGAAGGTGA TTACGGACGA AGAATTCCAA
      301 TTCATTATGA GATGTTTGGC AATTACACTA TCTCCTGTGA ACCACTAGAG ATAAATTGTC
      361 AGGCTCCACC AGTAGGTAGT CTAATCGTTC GTTGTTCATA CGATTATGAC TTTGTTGAGC
      421 ATCATGACGT TCGTGTTGTT CTAGATTTCA TCTAActcga ggcggccgc
//