LOCUS       pOME0064       467 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  HKU3 ORF 8a nt 27723-28088 tagged; vector pDEST47
SOURCE      HKU3
  ORGANISM  HKU3
            HKU3 ORF 8a tagged; UNC160-20; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            Plasmid received from RG tagged
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..450
                     /cds_type=ORF
                     /note="Length: 432"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            382..447
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            448..453
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(454..459,454^455)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 454^455"
                     /note="cut_0_on_negative_strand: 458^459"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            454..467
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(460..467,461^462)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 461^462"
                     /note="cut_0_on_negative_strand: 465^466"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GAAACTTCTC ATTGTTTTTG GACTCTTAGC ATCAGTGTAC
       61 TGCTTCCACA GAGAATGCAG CATACAAGAG TGTTGTGAAA ATCAACCCTA CCAAATTGAA
      121 GACCCATGTC CAATACATTA CTATTCGGAC TGGTTTATAA AAATTGGATC TAGAAAATCG
      181 GCTCGCCTCG TACAATTGTG CGAAGGTGAT TACGGACGAA GAATTCCAAT TCATTATGAG
      241 ATGTTTGGCA ATTACACTAT CTCCTGTGAA CCACTAGAGA TAAATTGTCA GGCTCCACCA
      301 GTAGGTAGTC TAATCGTTCG TTGTTCATAC GATTATGACT TTGTTGAGCA TCATGACGTT
      361 CGTGTTGTTC TAGATTTCAT Cgactacaaa gaccatgacg gtgattataa agatcatgat
      421 atcgattaca aggatgacga tgacaagtag taactcgagg cggccgc
//