LOCUS       pOME0065N       391 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  HKU3 ORF 9b nt 28113-28406 tagged; vector pDEST47
SOURCE      HKU3
  ORGANISM  HKU3
            HKU3 ORF 9b tagged; UNC163-9; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of Orfeome project
            "N" in name= N terminus 3x FLAG tag
            Internal XhoI site; so only NotI site at 3' end
            Plasmid received from RG
FEATURES             Location/Qualifiers
     Site            1..17
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(6..11,6^7)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 6^7"
                     /note="cut_0_on_negative_strand: 10^11"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            join(12..17,16^17)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 16^17"
                     /note="cut_0_on_negative_strand: 12^13"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            18..23
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             24..383
                     /cds_type=ORF
                     /note="Length: 360"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            27..92
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            join(384..391,385^386)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 385^386"
                     /note="cut_0_on_negative_strand: 389^390"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
     Site            384..391
                     /site_type="mutagenized site"
                     /label="3' RE tag"
ORIGIN
        1 TCGCCGTCGA CGGTACCGCC ACCATGGACT ACAAAGACCA TGACGGTGAT TATAAAGATC
       61 ATGATATCGA TTACAAGGAT GACGATGACA AGGACCCCAA AGTCAACGTA GTGCCCCCCG
      121 CATTACATTT GGTGGACCCG CAGATTCAAA TGACAATAAC CAGGATGGAG GACGCAGTGG
      181 TGCACGGCCA AAACAACGCC GGCCCCAAGG TTTACCCAAT AATACTGCGT CTTGGTTCAC
      241 AGCTCTCACT CAGCATGGCA AAGAGGAACT TAGATTCCCT CGAGGCCAGG GCGTTCCAAT
      301 CAACACCAAT AGTGGTAAAG ATGACCAAAT TGGCTACTAC CGAAGAGCTA CCCGACGAGT
      361 TCGTGGTGGT GACGGCAAAA TGAgcggccg c
//