LOCUS       pOME0066       389 bp    DNA     linear   UNA 23-Oct-2013
DEFINITION  HKU3 ORF 9b nt 28113-28406 tagged; vector pDEST47
SOURCE      HKU3
  ORGANISM  HKU3
            HKU3 ORF 9b tagged; 163-10; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesized by BioBasic
            Sequencce verified via Sanger sequencing through Genewiz
            pOME= plasmid of Orfeome project
            Internal XhoI site; so only NotI site at 3' end
            Plasmid received from RG tagged
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..378
                     /cds_type=ORF
                     /note="Length: 360"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            310..375
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            376..381
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(382..389,383^384)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 383^384"
                     /note="cut_0_on_negative_strand: 387^388"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
     Site            382..389
                     /site_type="mutagenized site"
                     /label="3' RE tag"
ORIGIN
        1 gtcgacggta ccgccaccAT GGACCCCAAA GTCAACGTAG TGCCCCCCGC ATTACATTTG
       61 GTGGACCCGC AGATTCAAAT GACAATAACC AGGATGGAGG ACGCAGTGGT GCACGGCCAA
      121 AACAACGCCG GCCCCAAGGT TTACCCAATA ATACTGCGTC TTGGTTCACA GCTCTCACTC
      181 AGCATGGCAA AGAGGAACTT AGATTCCCTC GAGGCCAGGG CGTTCCAATC AACACCAATA
      241 GTGGTAAAGA TGACCAAATT GGCTACTACC GAAGAGCTAC CCGACGAGTT CGTGGTGGTG
      301 ACGGCAAAAg actacaaaga ccatgacggt gattataaag atcatgatat cgattacaag
      361 gatgacgatg acaagtagta agcggccgc
//