LOCUS pOME0066 389 bp DNA linear UNA 23-Oct-2013 DEFINITION HKU3 ORF 9b nt 28113-28406 tagged; vector pDEST47 SOURCE HKU3 ORGANISM HKU3 HKU3 ORF 9b tagged; 163-10; Synthetic Construct. REFERENCE 1 (bases 1 to 887) AUTHORS Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P., Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y., Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and Texier,J.R. TITLE Unpublished COMMENT Plasmid synthesized by BioBasic Sequencce verified via Sanger sequencing through Genewiz pOME= plasmid of Orfeome project Internal XhoI site; so only NotI site at 3' end Plasmid received from RG tagged FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..378 /cds_type=ORF /note="Length: 360" /note="Found at strand: positive" /note="Start codon: ATG" Site 310..375 /site_type="mutagenized site" /label="3X FLAG tag" Site 376..381 /site_type="mutagenized site" /label="double stop" Site join(382..389,383^384) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 383^384" /note="cut_0_on_negative_strand: 387^388" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI Site 382..389 /site_type="mutagenized site" /label="3' RE tag" ORIGIN 1 gtcgacggta ccgccaccAT GGACCCCAAA GTCAACGTAG TGCCCCCCGC ATTACATTTG 61 GTGGACCCGC AGATTCAAAT GACAATAACC AGGATGGAGG ACGCAGTGGT GCACGGCCAA 121 AACAACGCCG GCCCCAAGGT TTACCCAATA ATACTGCGTC TTGGTTCACA GCTCTCACTC 181 AGCATGGCAA AGAGGAACTT AGATTCCCTC GAGGCCAGGG CGTTCCAATC AACACCAATA 241 GTGGTAAAGA TGACCAAATT GGCTACTACC GAAGAGCTAC CCGACGAGTT CGTGGTGGTG 301 ACGGCAAAAg actacaaaga ccatgacggt gattataaag atcatgatat cgattacaag 361 gatgacgatg acaagtagta agcggccgc //