LOCUS pOME0086 413 bp DNA linear UNA 23-Oct-2013 DEFINITION MERS CoV England ORF 3 tagged nt 25531-25842; vector pDEST47 SOURCE MERS ORGANISM MERS MERS CoV England ORF 3 tagged; UNC163-12; Synthetic Construct. REFERENCE 1 (bases 1 to 887) AUTHORS Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P., Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y., Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and Texier,J.R. TITLE Unpublished COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of Orfeome project Plasmid received from RG FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..396 /cds_type=ORF Site 328..393 /site_type="mutagenized site" /label="3X FLAG tag" Site 394..399 /site_type="mutagenized site" /label="double stop" Site join(400..405,400^401) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 400^401" /note="cut_0_on_negative_strand: 404^405" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 400..413 /site_type="mutagenized site" /label="3' RE tag" Site join(406..413,407^408) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 407^408" /note="cut_0_on_negative_strand: 411^412" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccAT GAGAGTTCAA AGACCACCCA CTCTCTTGTT AGTGTTCTCA 61 CTCTCTCTTT TGGTCACTGC ATTCTCAAAA CCTCTCTATG TACCTGAGCA TTGTCAGAAT 121 TATTCTGGTT GCATGCTTAG GGCTTGTATT AAAACTGCCC AAGCTGATAC AGCTGGTCTT 181 TATACAAATT TTCGAATTGA CGTCCCATCT GCAGAATCAA CTGGTACTCA ATCAGTTTCT 241 GTCGATCGTG AGTCAACTTC AACTCATGAT GGTCCTACCG AACATGTTAC TAGTGTGAAT 301 CTTTTTGACA TTGGTTACTC AGTTAATgac tacaaagacc atgacggtga ttataaagat 361 catgatatcg attacaagga tgacgatgac aagtagtaac tcgaggcggc cgc //