LOCUS       pOME0097       350 bp    DNA     linear   UNA 14-Nov-2013
DEFINITION  ZEBOV NP Hypo #3 Tagged; pDEST47
SOURCE      ZEBOV
  ORGANISM  ZEBOV
            ZEBIV NP Hypo #3 tagged; UNC161-4; Synthetic Construct.
REFERENCE   1 (bases 1 to 887)
  AUTHORS   Baric,R.S., Corcoran,K., Damania,B., Dittmer,D.P.,
            Graham,R.L., Halfmann,P., Heise,M.T., Kawaoka,Y.,
            Long,K.M., Moorman,N.J., Neumann,G., Sherrod,C.J., and
            Texier,J.R.
  TITLE     Unpublished
COMMENT     Plasmid synthesizthed by BioBasic 
            Sequence verified via Sanger sequencing
            through Genewiz
            pOME= plasmid of Orfeome project
            "Hypo"= hypothetical gene
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..333
                     /cds_type=ORF
                     /note="Length: 315"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            265..330
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            331..336
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(337..342,337^338)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 337^338"
                     /note="cut_0_on_negative_strand: 341^342"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            337..350
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(343..350,344^345)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 344^345"
                     /note="cut_0_on_negative_strand: 348^349"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GCCGACGACG AGACGTCTAG CCTTCCGCCC TTGGAGTCAG
       61 ATGATGAAGA GCAGGACAGG GACGGAACTT CCAACCGCAC ACCCACTGTC GCCCCACCGG
      121 CTCCCGTATA CAGAGATCAC TCTGAAAAGA AAGAACTCCC GCAAGACGAG CAACAAGATC
      181 AGGACCACAC TCAAGAGGCC AGGAACCAGG ACAGTGACAA CACCCAGTCA GAACACTCTT
      241 TTGAGGAGAT GTATCGCCAC ATTCgactac aaagaccatg acggtgatta taaagatcat
      301 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc
//