LOCUS       pOME0099       437 bp    DNA     linear   UNA 14-Nov-2013
DEFINITION  ZEBOV GP Hypo #1 tagged; vector pDEST47
            
SOURCE      UNC 161-6 (ZEBOV GP Hypo #1 tagged)
COMMENT     Synthesized by BioBasic 
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid ofthe Orfeome project
            ZEBOV= Zaire EBOLA virus
            Hypo= Hypothetical gene
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..420
                     /cds_type=ORF
                     /note="Length: 402"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            352..417
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            418..423
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(424..429,424^425)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 424^425"
                     /note="cut_0_on_negative_strand: 428^429"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            424..437
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(430..437,431^432)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 431^432"
                     /note="cut_0_on_negative_strand: 435^436"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GGAGTGGCAA CTGACGTGCC ATCTGCAACT AAAAGATGGG
       61 GCTTCAGGTC CGGTGTCCCA CCAAAGGTGG TCAATTATGA AGCTGGTGAA TGGGCTGAAA
      121 ACTGCTACAA TCTTGAAATC AAAAAACCTG ACGGGAGTGA GTGTCTACCA GCAGCGCCAG
      181 ACGGGATTCG GGGCTTCCCC CGGTGCCGGT ATGTGCACAA AGTATCAGGA ACGGGACCGT
      241 GTGCCGGAGA CTTTGCCTTC CATAAAGAGG GTGCTTTCTT CCTGTATGAT CGACTTGCTT
      301 CCACAGTTAT CTACCGAGGA ACGACTTTCG CTGAAGGTGT CGTTGCATTT Cgactacaaa
      361 gaccatgacg gtgattataa agatcatgat atcgattaca aggatgacga tgacaagtag
      421 taactcgagg cggccgc
//