LOCUS       pOME0101       860 bp    DNA     linear   UNA 14-Nov-2013
DEFINITION  A/WSN/33 PA-X tagged; vector pDEST47
SOURCE      UNC 161-8 (A/WSN/33 PA-X tagged)
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            A/WSN/33 PA-X= Influenza type/Wisconsin/isolated in 1933/PA-X gene
            
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..843
                     /cds_type=ORF
                     /note="Length: 825"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            775..840
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            841..846
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(847..852,847^848)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 847^848"
                     /note="cut_0_on_negative_strand: 851^852"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            847..860
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(853..860,854^855)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 854^855"
                     /note="cut_0_on_negative_strand: 858^859"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GGAAGATTTT GTGCGACAAT GCTTCAATCC GATGATTGTC
       61 GAGCTTGCGG AAAAGGCAAT GAAAGAGTAT GGAGAGGACC TGAAAATCGA AACAAACAAA
      121 TTTGCAGCAA TATGCACTCA CTTGGAAGTG TGCTTCATGT ATTCAGATTT TCACTTCATC
      181 GATGAGCAAG GCGAGTCAAT AGTCGTAGAA CTTGGCGATC CAAATGCACT TTTGAAGCAC
      241 AGATTTGAAA TAATCGAGGG AAGAGATCGC ACAATAGCCT GGACAGTAAT AAACAGTATT
      301 TGCAACACTA CAGGGGCTGA GAAACCAAAG TTTCTACCAG ATTTGTATGA TTACAAGAAG
      361 AATAGATTCA TCGAAATTGG AGTAACAAGG AGAGAAGTTC ACATATACTA TCTGGAAAAG
      421 GCCAATAAAA TTAAATCTGA GAAGACACAC ATCCACATTT TCTCATTCAC TGGGGAGGAA
      481 ATGGCCACAA AGGCCGACTA CACTCTCGAT GAAGAAAGCA GGGCTAGGAT CAAAACCAGG
      541 CTATTCACCA TAAGACAAGA AATGGCTAGC AGAGGCCTCT GGGATTCCTT TGTCAGTCCG
      601 AGAGAGGCGA AGAGACAATT GAAGAAAGAT TTGAAATCAC AGGAACAATG CGCAAGCTTG
      661 CCGACCAAAG TCTCCCGCCA AACTTCTCCA GCCTTGAAAA ATTTAGAGCC TATGTGGATG
      721 GATTCGAACC GAACGGCTAC ATTGAGGGCA AGCTTTCTCA AATGTCCAAA GAAGgactac
      781 aaagaccatg acggtgatta taaagatcat gatatcgatt acaaggatga cgatgacaag
      841 tagtaactcg aggcggccgc
//