LOCUS       pOME0103       308 bp    DNA     linear   UNA 14-Nov-2013
DEFINITION  A/Brevig Mission 1/1918 M3 tagged; vector pDEST47
SOURCE      UNC161-10 (A/Brevig Mission 1/1918 M3 tagged)
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            A/Brevig Mission/1/1918 M3= Influenza type A/Brevig Mission/isolated in 1918/M3 gene
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..291
                     /cds_type=ORF
                     /note="Length: 273"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            223..288
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            289..294
                     /site_type="mutagenized site"
                     /label="double s top"
     Site            join(295..300,295^296)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 295^296"
                     /note="cut_0_on_negative_strand: 299^300"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            295..308
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(301..308,302^303)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 302^303"
                     /note="cut_0_on_negative_strand: 306^307"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GACCATCGTC AACATCCACA GCACTCTGCT GTTCCTTTCG
       61 ATATTCTTCC CTCATAGACT CCGGCACTCC TTCCGTAGAA GGCCCTCTTT TCAAACCGTA
      121 TTTAAGGCGA CGATAAATGC ATTTGAAAAA AAGACGATCA AGAATCCACA ATATCAAGTG
      181 CAAGATCCCA ATGATACTTG CGGCAATAAC GAGAGGATCA CTgactacaa agaccatgac
      241 ggtgattata aagatcatga tatcgattac aaggatgacg atgacaagta gtaactcgag
      301 gcggccgc
//