LOCUS       pOME0104       854 bp    DNA     linear   UNA 14-Nov-2013
DEFINITION  EBOV-Bat VP24 tagged; vector pDEST47
SOURCE      UNC161-11 (EBOV-Bat VP24 tagged)
COMMENT     Non-tagged version already exists from Genescript; only ordering tagged version
            Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            EBOV-Bat= Bat Ebola virus 
            pOME= plasmid of the Orfeome project
            VP= viral protein
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..837
                     /cds_type=ORF
                     /note="Length: 819"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            769..834
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            835..840
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(841..846,841^842)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 841^842"
                     /note="cut_0_on_negative_strand: 845^846"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            841..854
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(847..854,848^849)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 848^849"
                     /note="cut_0_on_negative_strand: 852^853"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GGCCCGACCG TCAGGAAGGT ACAACATCCT CCAGACCGAC
       61 ACATTCCATG AGAATACTGT TGTAAAACAT GATCTATGTA ATTTTCTTGT CACAACAACA
      121 ATCACTGGGT GGGATGTCTA CTGGGCTGGT CATCTTTTCC ATGTCCCCAA CAAAGGAATT
      181 GCGCTTCTCA CCCGTCTAAA GACATCTGAT TTTGCACCTG CATGGACCCT GACCAAGAAT
      241 CTATTTCCAC ATCTATTTCA TAACCCGACC AGTACCCTCA AAGACCCTGT TTGGGGCCTT
      301 AGAGTGATCC TGGCAGCAGC CCTGTATGAT CAAGTAATGG AACAATCCCT CATCAAACCC
      361 CTAAGCGAGA CCTTAACTCT GCTAGGAGAT TGGCTTTTGA CCACAGAAAC GCAAAAATTC
      421 AATCTGAGAA CCCCTCGGGC CCAGGAACAA ATAAGCTTGA GGATGTTATC CTTGGTAAAG
      481 AAGCATGTGG ACAACTTTAT CCTAAAACTT CTTGAACTTC ATGTCATAAA CAATCGAGGG
      541 TTTACAAGCA GCATTGAAAT CGGAAACAAG TGTAATACTA TAATCATTTC CCGAACAAAC
      601 ATGGGATACC TTGTTGAACA GCAGGAACCG GACAAGTCTG CACTTCGAAA AACAAAACCT
      661 GGTCCAGTCA AATTTACTTT AGTGCACGAG GATGCATTCA GGGACTTTAA ACCAGAAAGA
      721 TCGAGCATAA ATTTATTAAT CATGGAGTTT AATAGTTCAC TCGCCATAga ctacaaagac
      781 catgacggtg attataaaga tcatgatatc gattacaagg atgacgatga caagtagtaa
      841 ctcgaggcgg ccgc
//