LOCUS       pOME0105      1064 bp    DNA     linear   UNA 14-Nov-2013
DEFINITION  EBOV-Bat VP35 tagged; vector pDEST47
SOURCE      UNC161-12 (EBOV-Bat VP35 tagged)
COMMENT     Already exists from Genescript; will only be synthesizing the tagged version
            Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            EBOV-Bat= Bat Ebola virus
            VP= viral protein
            
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..1047
                     /cds_type=ORF
                     /note="Length: 1029"
                     /note="Found at strand: positive"
                     /note="Start codon: Atg"
     Site            979..1044
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            1045..1050
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(1051..1056,1051^1052)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 1051^1052"
                     /note="cut_0_on_negative_strand: 1055^1056"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            1051..1064
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(1057..1064,1058^1059)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 1058^1059"
                     /note="cut_0_on_negative_strand: 1062^1063"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAt ggacaaagtg acggatgcca tcatgactgg gcgattatca
       61 ctcgacacaa tccctggctt tcaatgctct gcgccgacct ccaggagcag gcccaccggg
      121 cgaaagtgtc caccgtggga aggaccactc attgagccca agacttccgc caacaaatca
      181 acacaaactg agaacatcta ccaaagcgac caggtcctac gcgaaatcaa acaactgagc
      241 tcaattctag cccaccaaat cacaaccatg gagaggctag agggttcact tcaaagcata
      301 caccaacagc ttcaggtggt cgggggcatg gcacataacc taacatcctt gacaaccctg
      361 tgcacagaaa tggttgccaa atatgacttc cttgtcatga ccaccggacg agcaactgca
      421 acagcggcgg caacagaggc ttactggaaa gaacatggga aagccccacc tggcccagcc
      481 ttgttcgaag cagatgcact taaggcacga atccaagaaa atcccaaaag tgtcccgact
      541 gatgttagag aggcctttga ccgtctagag aagactgagg aggtaacaga gaggacgttc
      601 ggaaagccaa ccatctcagc taagctcctc aaggaacttc tctatgatca tcttcctgga
      661 tatgggactg ccttccatca gcttgcccaa gtcgtatgca aggtcgggaa agacaatgat
      721 ctcctggata tcatacatgc tgagttccag gccagcctag cagagggtga ctctccgcaa
      781 tgtgccctga ttcagatcac gaagcgaatc cccatcttcg gggagacacc acctcccgtc
      841 atctacataa aaacaaaaca agaaataccc aaagcatgcc aaaaaagcct ccgccctctt
      901 ccccccaatc ctaaaattga acggggctgg gtatgccatt atgtaacagc agatggaaag
      961 gaaatgggcc tcaagataga ctacaaagac catgacggtg attataaaga tcatgatatc
     1021 gattacaagg atgacgatga caagtagtaa ctcgaggcgg ccgc
//