LOCUS       pOME0107      1190 bp    DNA     linear   UNA 14-Nov-2013
DEFINITION  EBOV sGP tagged; vector pDEST47
SOURCE      UNC161-14 (EBOV sGP tagged)
COMMENT     Internal Sal site;per Mark make tag and untagged although monoclonal exists
            Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            sGP= soluble glycoprotein
            EBOV= Ebola virus
            
FEATURES             Location/Qualifiers
     Site            join(1..6,5^6)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 5^6"
                     /note="cut_0_on_negative_strand: 1^2"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             13..1173
                     /cds_type=ORF
                     /note="Length: 1161"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            1105..1170
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            1171..1176
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(1177..1182,1177^1178)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 1177^1178"
                     /note="cut_0_on_negative_strand: 1181^1182"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            1177..>1190
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(1183..1190,1184^1185)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 1184^1185"
                     /note="cut_0_on_negative_strand: 1188^1189"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 ggtaccgcca ccATGGGCGT TACAGGAATA TTGCAGTTAC CTCGTGATCG ATTCAAGAGG
       61 ACATCATTCT TTCTTTGGGT AATTATCCTT TTCCAAAGAA CATTTTCCAT CCCACTTGGA
      121 GTCATCCACA ATAGCACATT ACAGGTTAGT GATGTCGACA AACTAGTTTG TCGTGACAAA
      181 CTGTCATCCA CAAATCAATT GAGATCAGTT GGACTGAATC TCGAAGGGAA TGGAGTGGCA
      241 ACTGACGTGC CATCTGCAAC TAAAAGATGG GGCTTCAGGT CCGGTGTCCC ACCAAAGGTG
      301 GTCAATTATG AAGCTGGTGA ATGGGCTGAA AACTGCTACA ATCTTGAAAT CAAAAAACCT
      361 GACGGGAGTG AGTGTCTACC AGCAGCGCCA GACGGGATTC GGGGCTTCCC CCGGTGCCGG
      421 TATGTGCACA AAGTATCAGG AACGGGACCG TGTGCCGGAG ACTTTGCCTT CCATAAAGAG
      481 GGTGCTTTCT TCCTGTATGA TCGACTTGCT TCCACAGTTA TCTACCGAGG AACGACTTTC
      541 GCTGAAGGTG TCGTTGCATT TCTGATACTG CCCCAAGCTA AGAAGGACTT CTTCAGCTCA
      601 CACCCCTTGA GAGAGCCGGT CAATGCAACG GAGGACCCGT CTAGTGGCTA CTATTCTACC
      661 ACAATTAGAT ATCAGGCTAC CGGTTTTGGA ACCAATGAGA CAGAGTACTT GTTCGAGGTT
      721 GACAATTTGA CCTACGTCCA ACTTGAATCA AGATTCACAC CACAGTTTCT GCTCCAGCTG
      781 AATGAGACAA TATATACAAG TGGGAAAAGG AGCAATACCA CGGGAAAACT AATTTGGAAG
      841 GTCAACCCCG AAATTGATAC AACAATCGGG GAGTGGGCCT TCTGGGAAAC TAAAAAAACC
      901 TCACTAGAAA AATTCGCAGT GAAGAGTTGT CTTTCACAGT TGTATCAAAC GGAGCCAAAA
      961 ACATCAGTGG TCAGAGTCCG GCGCGAACTT CTTCCGACCC AGGGACCAAC ACAACAACTG
     1021 AAGACCACAA AATCATGGCT TCAGAAAATT CCTCTGCAAT GGTTCAAGTG CACAGTCAAG
     1081 GAAGGGAAGC TGCAGTGTCG CATCgactac aaagaccatg acggtgatta taaagatcat
     1141 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc
//