LOCUS       pOME0108       782 bp    DNA     linear   UNA 14-Nov-2013
DEFINITION  Vaccinia F1L tagged; vector pDEST47
            
SOURCE      UNC161-15 (Vaccinia F1L tagged)
COMMENT     Only tagged version; positive control only
            Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            EBOV= Ebola virus
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..765
                     /cds_type=ORF
                     /note="Length: 747"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            697..762
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            763..768
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(769..774,769^770)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 769^770"
                     /note="cut_0_on_negative_strand: 773^774"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            769..782
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(775..782,776^777)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 776^777"
                     /note="cut_0_on_negative_strand: 780^781"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GTTGTCGATG TTTATGTGTA ATAATATCGT AGATTATGTA
       61 GATGATATAG ATAATGGTAT AGTACAGGAT ATAGAAGATG AGGCTAGCAA TAATGTTGAT
      121 CACGACTATG TATATCCACT TCCAGAAAAT ATGGTATATA GATTTGACAA GTCCACTAAC
      181 ATACTCGATT ATCTATCAAC GGAACGGGAC CATGTAATGA TGGCTGTTCG ATACTATATG
      241 AGTAAACAAC GTTTAGACGA CTTGTATAGA CAGTTGCCCA CAAAGACTAG ATCATATATA
      301 GATATTATCA ACATATATTG TGATAAAGTT AGTAATGATT ATAATAGGGA CATGAATATC
      361 ATGTATGATA TGGCATCTAC AAAATCATTT ACAGTTTATG ACATAAATAA CGAAGTTAAT
      421 ACTATACTAA TGGATAACAA GGGGTTGGGT GTAAGATTGG CGACAATTTC ATTTATAACC
      481 GAATTGGGTA GACGATGTAT GAACCCAGTA GAAACGATAA AAATGTTTAC TCTACTATCG
      541 CATACTATAT GCGATGATTA TTTTGTAGAT TATATAACGG ACATTTCACC ACCAGATAAT
      601 ACCATCCCTA ACACTAGCAC GCGTGAATAT CTAAAGCTTA TTGGCATCAC AGCTATCATG
      661 TTTGCTACAT ATAAAACTCT CAAATACATG ATAGGAgact acaaagacca tgacggtgat
      721 tataaagatc atgatatcga ttacaaggat gacgatgaca agtagtaact cgaggcggcc
      781 gc
//