LOCUS pOME0108 782 bp DNA linear UNA 14-Nov-2013 DEFINITION Vaccinia F1L tagged; vector pDEST47 SOURCE UNC161-15 (Vaccinia F1L tagged) COMMENT Only tagged version; positive control only Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of the Orfeome project EBOV= Ebola virus FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..765 /cds_type=ORF /note="Length: 747" /note="Found at strand: positive" /note="Start codon: ATG" Site 697..762 /site_type="mutagenized site" /label="3X FLAG tag" Site 763..768 /site_type="mutagenized site" /label="double stop" Site join(769..774,769^770) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 769^770" /note="cut_0_on_negative_strand: 773^774" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 769..782 /site_type="mutagenized site" /label="3' RE tag" Site join(775..782,776^777) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 776^777" /note="cut_0_on_negative_strand: 780^781" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccAT GTTGTCGATG TTTATGTGTA ATAATATCGT AGATTATGTA 61 GATGATATAG ATAATGGTAT AGTACAGGAT ATAGAAGATG AGGCTAGCAA TAATGTTGAT 121 CACGACTATG TATATCCACT TCCAGAAAAT ATGGTATATA GATTTGACAA GTCCACTAAC 181 ATACTCGATT ATCTATCAAC GGAACGGGAC CATGTAATGA TGGCTGTTCG ATACTATATG 241 AGTAAACAAC GTTTAGACGA CTTGTATAGA CAGTTGCCCA CAAAGACTAG ATCATATATA 301 GATATTATCA ACATATATTG TGATAAAGTT AGTAATGATT ATAATAGGGA CATGAATATC 361 ATGTATGATA TGGCATCTAC AAAATCATTT ACAGTTTATG ACATAAATAA CGAAGTTAAT 421 ACTATACTAA TGGATAACAA GGGGTTGGGT GTAAGATTGG CGACAATTTC ATTTATAACC 481 GAATTGGGTA GACGATGTAT GAACCCAGTA GAAACGATAA AAATGTTTAC TCTACTATCG 541 CATACTATAT GCGATGATTA TTTTGTAGAT TATATAACGG ACATTTCACC ACCAGATAAT 601 ACCATCCCTA ACACTAGCAC GCGTGAATAT CTAAAGCTTA TTGGCATCAC AGCTATCATG 661 TTTGCTACAT ATAAAACTCT CAAATACATG ATAGGAgact acaaagacca tgacggtgat 721 tataaagatc atgatatcga ttacaaggat gacgatgaca agtagtaact cgaggcggcc 781 gc //