LOCUS       pOME0128       446 bp    DNA     linear   UNA 27-Nov-2013
DEFINITION  KSHV K4.1 GQ994935 TAGGED; vector pDEST47
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            KSHV=Kaposi's sarcoma-associated virus
            GQ994935= reference acceesion #
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak seuence"
     CDS             19..429
                     /cds_type=ORF
                     /note="Length: 411"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            361..426
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            427..432
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(433..438,433^434)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 433^434"
                     /note="cut_0_on_negative_strand: 437^438"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            433..446
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(439..446,440^441)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 440^441"
                     /note="cut_0_on_negative_strand: 444^445"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccat gtggagcatg tgctgggtgt tgcgcgcaca tttggggctg
       61 ctcttctggg tggccgtgat tgagctatgt gcggccagcg gtcctgccac catcatggcc
      121 tcggattgtt gcgagaattc cctgtccagt gccagattgc caccggataa gctgatatgc
      181 gggtggtact ggacgtccac cgtgtattgt cgccagaagg cagtcatttt tgtaacgcac
      241 tcggggcgga aagtatgcgg gtcgcctgcg aagcggcgca cgcgtcttct gatggagaag
      301 cacacggaga tacctctcgc gaagcgcgta gcgcttcgcg ccggtaaagg gttatgcccc
      361 gactacaaag accatgacgg tgattataaa gatcatgata tcgattacaa ggatgacgat
      421 gacaagtagt aactcgaggc ggccgc
//