LOCUS       pOME0142R       365 bp    DNA     linear   UNA 13-Jan-2014
DEFINITION  BatSARS Urbani RBD 10049-10312 TAGGED; vector pVR21
SOURCE      UNC166-18 (BatSARS Urbani RBD 10049-10312 TAGGED)
COMMENT     81 and 180 changed from A-->C to avoid internal orfs - does not change AA
            Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            RBD= receptor binding domain 
            R in plasmid name= in replicon pVR21
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..348
                     /cds_type=ORF
                     /note="Length: 330"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            280..345
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            346..351
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(352..357,352^353)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 352^353"
                     /note="cut_0_on_negative_strand: 356^357"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            352..365
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(358..365,359^360)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 359^360"
                     /note="cut_0_on_negative_strand: 363^364"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GGTTTGTGGT TGGATGACAC AGTGTACTGC CCAAGACATG
       61 TTGTTTGCAC GGCTGAAGAC cTGCTTAACC CGAACTATGA CGACCTGCTC ATCCGCAAAT
      121 CTAACCATAG TTTCCTTGTT CAAGCTGGTA ATGTCCAACT TCGTGTTATT GGCCATTCCc
      181 TGCAAAATTG TCTGCTTAGG CTTAAAGTTG ACACTTCTAA CCCTAAGACA CCAAAGTATA
      241 AATTTGTCCG TATCCAACCA GGTCAGACAT TTTCAGTCCg actacaaaga ccatgacggt
      301 gattataaag atcatgatat cgattacaag gatgacgatg acaagtagta actcgaggcg
      361 gccgc
//