LOCUS pOME0142R 365 bp DNA linear UNA 13-Jan-2014 DEFINITION BatSARS Urbani RBD 10049-10312 TAGGED; vector pVR21 SOURCE UNC166-18 (BatSARS Urbani RBD 10049-10312 TAGGED) COMMENT 81 and 180 changed from A-->C to avoid internal orfs - does not change AA Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of the Orfeome project RBD= receptor binding domain R in plasmid name= in replicon pVR21 FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..348 /cds_type=ORF /note="Length: 330" /note="Found at strand: positive" /note="Start codon: ATG" Site 280..345 /site_type="mutagenized site" /label="3X FLAG tag" Site 346..351 /site_type="mutagenized site" /label="double stop" Site join(352..357,352^353) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 352^353" /note="cut_0_on_negative_strand: 356^357" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 352..365 /site_type="mutagenized site" /label="3' RE tag" Site join(358..365,359^360) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 359^360" /note="cut_0_on_negative_strand: 363^364" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccAT GGTTTGTGGT TGGATGACAC AGTGTACTGC CCAAGACATG 61 TTGTTTGCAC GGCTGAAGAC cTGCTTAACC CGAACTATGA CGACCTGCTC ATCCGCAAAT 121 CTAACCATAG TTTCCTTGTT CAAGCTGGTA ATGTCCAACT TCGTGTTATT GGCCATTCCc 181 TGCAAAATTG TCTGCTTAGG CTTAAAGTTG ACACTTCTAA CCCTAAGACA CCAAAGTATA 241 AATTTGTCCG TATCCAACCA GGTCAGACAT TTTCAGTCCg actacaaaga ccatgacggt 301 gattataaag atcatgatat cgattacaag gatgacgatg acaagtagta actcgaggcg 361 gccgc //