LOCUS       pOME0146CO       389 bp    DNA     linear   UNA 13-Jan-2014
DEFINITION  Human codon optimized BatSARS Urbani RBD 28164-28457 TAGGED; vector pDEST47
            
SOURCE      UNC166-20 (Human codon optimized BatSARS Urbani RBD 28164-28457 TAGGED)
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            RBD= receptor binding domain 
            CO= codon optimized 
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..378
                     /cds_type=ORF
                     /note="Length: 360"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            310..375
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            376..381
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(382..389,383^384)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 383^384"
                     /note="cut_0_on_negative_strand: 387^388"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
     Site            382..389
                     /site_type="mutagenized site"
                     /label="3' RE tag"
ORIGIN
        1 gtcgacggta ccgccaccAT GGACCCTAAG GTCAACGTCG TGCCTCCAGC ACTTCACCTC
       61 GTGGACCCAC AAATCCAGAT GACCATCACA AGGATGGAGG ACGCTGTGGT GCATGGACAG
      121 AACAACGCCG GCCCCAAAGT GTACCCCATT ATCCTGAGAC TGGGGTCCCA GCTCTCTCTC
      181 TCAATGGCCA AGCGGAATTT GGATAGCCTG GAGGCTCGCG CCTTCCAGAG TACACCCATT
      241 GTGGTCAAGA TGACTAAGCT GGCCACTACC GAGGAGCTGC CCGATGAATT TGTCGTGGTG
      301 ACAGCCAAGg actacaaaga ccatgacggt gattataaag atcatgatat cgattacaag
      361 gatgacgatg acaagtagta agcggccgc
//