LOCUS       pOME0148       591 bp    DNA     linear   UNA 13-Jan-2014
DEFINITION  SARS CoV MA 734-1225; vector pDEST47
SOURCE      UNC166-22 (SARS CoV MA 734-1225)
COMMENT     Need XmnI site at 5' end due to internal Kpn and Sal sites.
            A-->G at 165 to stop internal orf; no aa change
            Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            
FEATURES             Location/Qualifiers
     Site            join(1..10,5^6)
                     /site_type="restriction site"
                     /note="Name: XmnI"
                     /note="Pattern: gaannnnttc"
                     /note="cut_0_on_positive_strand: 5^6"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XmnI
     Site            1..10
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            11..16
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             17..574
                     /cds_type=ORF
                     /note="Length: 558"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            506..571
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            join(578..583,578^579)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 578^579"
                     /note="cut_0_on_negative_strand: 582^583"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            578..591
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(584..591,585^586)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 585^586"
                     /note="cut_0_on_negative_strand: 589^590"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gaaccaattc gccaccATGA ACAAAACTGG AACACTAAGC ATGGCAGTGG TGCACTCCGT
       61 GAACTCACTC GTGAGCTCAA TGGAGGTGCA GTCACTCGCT ATGTCGACAA CAATTTCTGT
      121 GGCCCAGATG GGTACCCTCT TGATTGCATC AAAGATTTTC TCGCACGCGC GGGCAAGTCA
      181 gTGTGCACTC TTTCCGAACA ACTTGATTAC ATCGAGTCGA AGAGAGGTGT CTACTGCTGC
      241 CGTGACCATG AGCATGAAAT TGCCTGGTTC ACTGAGCGCT CTGATAAGAG CTACGAGCAC
      301 CAGACACCCT TCGAAATTAA GAGTGCCAAG AAATTTGACA CTTTCAAAGG GGAATGCCCA
      361 AAGTTTGTGT TTCCTCTTAA CTCAAAAGTC AAAGTCATTC AACCACGTGT TGAAAAGAAA
      421 AAGACTGAGG GTTTCATGGG GCGTATACGC TCTGTGTACC CTGTTGCATC TCCACAGGAG
      481 TGTAACAATA TGCACTTGTC TACCTgacta caaagaccat gacggtgatt ataaagatca
      541 tgatatcgat tacaaggatg acgatgacaa gtagtaactc gaggcggccg c
//