LOCUS       pOME0148CO       591 bp    DNA     linear   UNA 13-Jan-2014
DEFINITION  Human codon optimized SARS CoV MA 734-1225 tagged; vector pDEST47
SOURCE      UNC166-22 (Human codon optimized SARS CoV MA 734-1225)
COMMENT     Need XmnI site at 5' end due to internal Kpn and Sal sites.
            A-->G at 165 to stop internal orf; no aa change
            Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            CO= codon optimized
FEATURES             Location/Qualifiers
     Site            join(1..10,5^6)
                     /site_type="restriction site"
                     /note="Name: XmnI"
                     /note="Pattern: gaannnnttc"
                     /note="cut_0_on_positive_strand: 5^6"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XmnI
     Site            1..10
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            11..16
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             17..574
                     /cds_type=ORF
                     /note="Length: 558"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            506..571
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            572..577
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(578..583,578^579)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 578^579"
                     /note="cut_0_on_negative_strand: 582^583"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            578..591
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(584..591,585^586)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 585^586"
                     /note="cut_0_on_negative_strand: 589^590"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gaaccaattc gccaccATGA ACAAGACCGG AACCCTCAGC ATGGCAGTGG TGCATAGCGT
       61 GAACTCCCTC GTGTCCAGCA TGGAAGTCCA GTCCCTCGCT ATGTCCACCA CAATCTCCGT
      121 GGCTCAAATG GGGACCCTCC TTATCGCCTC TAAGATCTTC TCCCACGCTC GGGCCAGTCA
      181 GTGCGCCCTT TTTCCTAACA ACCTGATTAC CTCCAGCCGC CGGGAGGTGT CTACTGCCGC
      241 CGTGACAATG AGTATGAAGC TCCCTGGCTC TTTGTCAGCC TTGATTCGGG CCACATCAAC
      301 CAGACACCCA AGCAAGCTGA GAGTGCCCAG GAACTTGACA CTGAGCAAGG GGAATGCCCA
      361 GAGCCTGTGC TTCCTGCTGA CTCAGAAGAG CAAGTCCTTT AACCACGTCC TGAAAAGGAA
      421 ACGGCTGAGG GTCAGCTGGG GCGTCTACGC CCTGTGTACT CTGCTGCATC TCCACAGGAG
      481 CGTCACAATT TGCACATGCC TGCCCgacta caaagaccat gacggtgatt ataaagatca
      541 tgatatcgat tacaaggatg acgatgacaa gtagtaactc gaggcggccg c
//