LOCUS       pOME0150       404 bp    DNA     linear   UNA 13-Jan-2014
DEFINITION  SARS CoV MA 2993-3295 TAGGED; vector pDEST47
COMMENT     51 A-->C to avoid internal orf; no aa change
            Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..387
                     /cds_type=ORF
                     /note="Length: 369"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            319..384
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            385..390
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(391..396,391^392)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 391^392"
                     /note="cut_0_on_negative_strand: 395^396"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            391..404
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(397..404,398^399)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 398^399"
                     /note="cut_0_on_negative_strand: 402^403"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GATGCTGGTG AAGAAAACTT TTCATCACGT cTGTATTGTT
       61 CCTTTTACCC TCCAGATGAG GAAGAAGAGG ACGATGCAGA GTGTGAGGAA GAAGAAATTG
      121 ATGAAACCTG TGAACATGAG TACGGTACAG AGGATGATTA TCAAGGTCTC CCTCTGGAAT
      181 TTGGTGCCTC AGCTGAAACA GTTCGAGTTG AGGAAGAAGA AGAGGAAGAC TGGCTGGATG
      241 ATACTACTGA GCAATCAGAG ATTGAGCCAG AACCAGAACC TACACCTGAA GAACCAGTTA
      301 ATCAGTTTAC TGGTTATTga ctacaaagac catgacggtg attataaaga tcatgatatc
      361 gattacaagg atgacgatga caagtagtaa ctcgaggcgg ccgc
//