LOCUS       pOME0152CO       365 bp    DNA     linear   UNA 13-Jan-2014
DEFINITION  Human codon optimized SARS CoV MA 10067-10330 TAGGED; vector pDEST47
SOURCE      UNC166-24 (Human codon optimized SARS CoV MA 10067-10330 TAGGED)
COMMENT     63 and 162 A-->G to avoid internal orfs; no aa change
            Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..348
                     /cds_type=ORF
                     /note="Length: 330"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            280..345
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            346..351
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(352..357,352^353)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 352^353"
                     /note="cut_0_on_negative_strand: 356^357"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            352..365
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(358..365,359^360)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 359^360"
                     /note="cut_0_on_negative_strand: 363^364"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GGACTGCGGG TGGATGACAC AATACACTGT CCAGGATATG
       61 TCATTCGCAC AGCAGAAGAC ATGCCTTATC CTTACTATGA AGATCTGTAG CTTCGCCAAC
      121 CCTACCATCG CTTTCCTCTT TAGGCTGGCC ATGTTCAACT TTGTGCTGCT CGCCATCTTG
      181 TGCAAGATTG TCTGCTTGGG CCTGAAACTG ATTCTGCTGA CCCTCCGGCA TCCCAGTATT
      241 AATCTCTCTG TGTCCAACCT GGTGAAGCAC TTTCAGTTTg actacaaaga ccatgacggt
      301 gattataaag atcatgatat cgattacaag gatgacgatg acaagtagta actcgaggcg
      361 gccgc
//