LOCUS       pOME0154       290 bp    DNA     linear   UNA 13-Jan-2014
DEFINITION  SARS CoV MA 20121-20709 TAGGED; vector pDEST47
COMMENT     36 and 117 A-->G to prevent internal orfs; no aa change
            Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..273
                     /cds_type=ORF
                     /note="Length: 255"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            205..270
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            271..276
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(277..282,277^278)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 277^278"
                     /note="cut_0_on_negative_strand: 281^282"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            277..290
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(283..290,284^285)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 284^285"
                     /note="cut_0_on_negative_strand: 288^289"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GCTGAAATTT CATTCgTGCT TTGGTGTAAG GATGGACATG
       61 TTGAAACCTT CTACCCAAAA CTACAAGCAA GTCAAGCGTG GCAACCAGGT GTTGCGgTGC
      121 CTAACTTGTA CAAGATGCAA AGAATGCTTC TTGAAAAGTG TGACCTTCAG AATTATGGTG
      181 AAAATGCTGT TATACCAAAA GGAAgactac aaagaccatg acggtgatta taaagatcat
      241 gatatcgatt acaaggatga cgatgacaag tagtaactcg aggcggccgc
//