LOCUS       pOME0156CO       314 bp    DNA     linear   UNA 13-Jan-2014
DEFINITION  Human codon optimized SARS CoV MA 28583-28795 TAGGED; vector pDEST47
SOURCE      UNC166-26 (Human codon optimized SARS CoV MA 28583-28795 TAGGED)
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            CO= codon optimized
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..297
                     /cds_type=ORF
                     /note="Length: 279"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            229..294
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            295..300
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(301..306,301^302)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 301^302"
                     /note="cut_0_on_negative_strand: 305^306"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            301..314
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(307..314,308^309)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 308^309"
                     /note="cut_0_on_negative_strand: 312^313"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GCTGCCACCT TGCTACAACT TCCTCAAGGA GCAGCACTGC
       61 CAGAAGGCTT CCACACAGAG GGAGGCAGAG GCCGCCGTGA AGCCTCTCCT CGCTCCCCAT
      121 CACGTGGTGG CCGTGATTCA GGAAATCCAA CTGCTGGCCG CCGTCGGGGA GATCCTTTTG
      181 TTGGAGTGGC TGGCCGAGGT CGTCAAACTG CCCAGCCGGT ATTGTTGCga ctacaaagac
      241 catgacggtg attataaaga tcatgatatc gattacaagg atgacgatga caagtagtaa
      301 ctcgaggcgg ccgc
//