LOCUS       pOME0158      1703 bp    DNA     linear   UNA 14-Jan-2014
DEFINITION  CHIKV nsP1 from SriLankan isolate TAGGED; vector pDEST47
COMMENT     5' end only cut with SalI to avoid internal KpnI site 
            Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            CHIKV= Chickungunya Virus
            nsP= non-structural protein
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             13..1686
                     /cds_type=ORF
                     /note="Length: 1674"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     CDS             473..628
                     /cds_type=ORF
                     /note="Length: 156"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     CDS             1419..1601
                     /cds_type=ORF
                     /note="Length: 183"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            1618..1683
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            1684..1689
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(1690..1695,1690^1691)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 1690^1691"
                     /note="cut_0_on_negative_strand: 1694^1695"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            join(1696..1703,1697^1698)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 1697^1698"
                     /note="cut_0_on_negative_strand: 1701^1702"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacgcca ccatggatcc tgtgtacgtg gacatagacg ctgacagcgc ctttttgaag
       61 gccctgcaac gtgcgtaccc catgtttgag gtggaaccaa ggcaggtcac accgaatgac
      121 catgctaatg ctagagcgtt ctcgcatcta gctataaaac taatagagca ggaaattgac
      181 cccgactcaa ccatcctgga tatcggcagt gcgccagcaa ggaggatgat gtcggacagg
      241 aagtaccact gcgtctgccc gatgcgcagt gcggaagatc ccgagagact cgctaattat
      301 gcgagaaagc tagcatctgc cgcaggaaaa gtcctggaca gaaacatctc tggaaagatc
      361 ggggacttac aagcagtaat ggccgtgcca gacaaggaga cgccaacatt ctgcttacac
      421 acagacgtct catgtagaca gagagcagac gtcgctatat accaagacgt ctatgctgta
      481 cacgcaccca cgtcgctata ccaccaggcg attaaagggg tccgagtggc gtactgggtt
      541 gggttcgaca caaccccgtt catgtacaat gccatggcgg gtgcctaccc ctcatactcg
      601 acaaactggg cagatgagca ggtactgaag gctaagaaca taggattatg ttcaacagac
      661 ctgacggaag gtagacgagg caagttgtct attatgagag ggaaaaagct aaaaccgtgc
      721 gaccgtgtgc tgttctcagt agggtcaacg ctctacccgg aaagccgcga gctacttaag
      781 agctggcacc tgccatcggt gttccattta aagggcaaac tcagcttcac atgccgctgt
      841 gatacagtgg tttcgtgtga gggctacgtc gttaagagaa taacgatgag cccaggcctt
      901 tatggaaaaa ccacagggta tgcggtaacc caccacgcag acggattcct gatgtgcaag
      961 actaccgaca cggttgacgg cgaaagagtg tcattctcgg tgtgcacata cgtgccggcg
     1021 accatttgtg atcaaatgac cggcatcctt gctacagaag tcacgccgga ggatgcacag
     1081 aagctgttgg tggggctgaa ccagagaata gtggttaacg gcagaacgca acggaatatg
     1141 aacaccatga aaaattatct gcttcccgtg gtcgcccaag ccttcagtaa gtgggcaaag
     1201 gagtgccgga aagacatgga agatgaaaaa ctcctggggg tcagagaaag aacactgacc
     1261 tgctgctgtc tatgggcatt caagaagcag aaaacacaca cggtctacaa gaggccggat
     1321 acccagtcaa ttcagaaggt tcaggccgag tttgacagct ttgtggtacc gagtctgtgg
     1381 tcgtccgggt tgtcaatccc tttgaggact agaatcaaat ggttgttaag caaggtgcca
     1441 aaaaccgacc tgatcccata cagcggagac gcccgagaag cccgggacgc agaaaaagaa
     1501 gcagaggaag aacgagaagc agaactgact cgcgaagccc taccacctct acaggcagca
     1561 caggaagatg ttcaggtcga aatcgacgtg gaacagcttg aggacagagc gggcgcagac
     1621 tacaaagacc atgacggtga ttataaagat catgatatcg attacaagga tgacgatgac
     1681 aagtagtaac tcgaggcggc cgc
//