LOCUS       pOME0160      2495 bp    DNA     linear   UNA 17-Jan-2014
DEFINITION  CHIKV nsp2 Sri Lankan isolate TAGGED; vector pDEST47
COMMENT     5' end only cut with KpnI to avoid internal SalI sites
            Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            CHIKV= Chickungunya Virus
            nsP= non-structural protein
FEATURES             Location/Qualifiers
     Site            join(1..6,5^6)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 5^6"
                     /note="cut_0_on_negative_strand: 1^2"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            1..6
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            7..12
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             13..2478
                     /cds_type=ORF
                     /note="Length: 2466"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            13..15
                     /site_type="mutagenized site"
                     /label=Start
     CDS             738..854
                     /cds_type=ORF
                     /note="Length: 117"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            2410..2475
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            2476..2481
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(2482..2487,2482^2483)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 2482^2483"
                     /note="cut_0_on_negative_strand: 2486^2487"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            2482..2495
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(2488..2495,2489^2490)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 2489^2490"
                     /note="cut_0_on_negative_strand: 2493^2494"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 ggtaccgcca ccatgggaat aatagagact ccgagaggag ctatcaaagt tactgcccaa
       61 ccaacagacc acgtcgtggg agagtacctg gtactctccc cgcagaccgt actacgtagc
      121 cagaagctca gtctgattca cgctttggcg gagcaagtga agacgtgcac gcacaacgga
      181 cgagcaggga ggtatgcggt cgaagcgtac gacggccgag tcctagtgcc ctcaggctat
      241 gcaatctcgc ctgaagactt ccagagtcta agcgaaagcg caacgatggt gtataacgaa
      301 agagagttcg taaacagaaa gctacaccat attgcgatgc acggaccagc cctgaacacc
      361 gacgaagagt cgtatgagct ggtgagggca gagaggacag aacacgagta cgtctacgac
      421 gtggatcaga gaagatgctg taagaaggaa gaagccgcag gactggtact ggtgggcgac
      481 ttgactaatc cgccctacca cgaattcgca tatgaagggc taaaaatccg ccctgcctgc
      541 ccatacaaaa ttgcagtcat aggagtcttc ggagtaccgg gatctggcaa gtcagctatt
      601 atcaagaacc tagttaccag gcaggacctg gtgactagcg gaaagaaaga aaactgccaa
      661 gaaatcacca ccgacgtgat gagacagaga ggtctagaga tatctgcacg tacggttgac
      721 tcgctgctct tgaatggatg caacagacca gtcgacgtgt tgtacgtaga cgaggcgttt
      781 gcgtgccact ctggaacgct acttgctttg atcgccttgg tgagaccaag gcagaaagtt
      841 gtactttgtg gtgacccgaa gcagtgcggc ttcttcaata tgatgcagat gaaagtcaac
      901 tataatcaca acatctgcac ccaagtgtac cacaaaagta tctccaggcg gtgcacactg
      961 cctgtgaccg ccattgtgtc atcgttgcat tacgaaggca aaatgcgcac tacgaatgag
     1021 tacaacaagc cgattgtagt ggacactaca ggctcaacaa aacctgaccc tggagacctc
     1081 gtgttaacgt gcttcagagg gtgggttaaa caactgcaaa ttgactatcg tggatacgag
     1141 gtcatgacag cagccgcatc ccaagggtta accagaaaag gagtttacgc agttagacaa
     1201 aaagttaatg aaaacccgct ctatgcatca acgtcagagc acgtcaacgt actcctaacg
     1261 cgtacggaag gtaaactggt atggaagaca ctttccggcg acccgtggat aaagacgctg
     1321 cagaacccac cgaaaggaaa cttcaaagca actattaagg agtgggaggt ggagcatgca
     1381 tcaataatgg cgggcatctg cagtcaccaa atgaccttcg atacattcca aaataaagcc
     1441 aacgtttgtt gggctaagag cttggtccct atcctcgaaa cagcggggat aaaactaaat
     1501 gataggcagt ggtctcagat aattcaagcc ttcaaagaag acaaagcata ctcacctgaa
     1561 gtagccctga atgaaatatg tacgcgcatg tatggggtgg atctagacag cgggctattt
     1621 tctaaaccgt tggtgtctgt gtattacgcg gataaccact gggataatag gcctggaggg
     1681 aaaatgttcg gatttaaccc cgaggcagca tccattctag aaagaaagta cccattcaca
     1741 aaagggaagt ggaacatcaa caagcagatc tgcgtgacta ccaggaggat agaagacttt
     1801 aaccctacca ccaacatcat accggccaac aggagactac cacactcatt agtggccgaa
     1861 caccgcccag taaaagggga aagaatggaa tggctggtta acaagataaa cggccaccac
     1921 gtgctcctgg tcagtggcta taaccttgca ctgcctacta agagagtcac ttgggtagcg
     1981 ccgttaggtg cccgcggagc ggactacaca tacaacctag agttgggtct gccagcaacg
     2041 cttggtaggt atgaccttgt ggtcataaac atccacacac cttttcgcat acaccattac
     2101 caacagtgcg tcgaccacgc aatgaaactg caaatgctcg ggggtgactc attgagactg
     2161 ctcaaaccgg gcggctctct attgatcaga gcatatggtt acgcagatag aaccagtgaa
     2221 cgagtcatct gcgtattggg acgcaagttt agatcgtcta gagcgttgaa accaccatgt
     2281 gtcaccagca acactgagat gtttttccta ttcagcaact ttgacaatgg cagaaggaat
     2341 ttcacaactc atgtcatgaa caatcaactg aatgcagcct tcgtaggaca ggtcacccga
     2401 gcaggatgtg actacaaaga ccatgacggt gattataaag atcatgatat cgattacaag
     2461 gatgacgatg acaagtagta actcgaggcg gccgc
//