LOCUS       pOME0162      1676 bp    DNA     linear   UNA 17-Jan-2014
DEFINITION  CHIKV nsP3 early stop; missing 6 last amino acids; Sri Lankan isolate TAGGED; vector pDEST47
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            CHIKV= Chickungunya Virus
            nsP= non-structural protein
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..1659
                     /cds_type=ORF
                     /note="Length: 1641"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     CDS             701..826
                     /cds_type=ORF
                     /note="Length: 126"
                     /note="Found at strand: positive"
                     /note="Start codon: atg"
     Site            1591..1656
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            1657..1662
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(1663..1668,1663^1664)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 1663^1664"
                     /note="cut_0_on_negative_strand: 1667^1668"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            1663..1676
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(1669..1676,1670^1671)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 1670^1671"
                     /note="cut_0_on_negative_strand: 1674^1675"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccat ggcaccgtcg taccgggtaa aacgcatgga catcgcgaag
       61 aacgatgaag agtgcgtagt caacgccgct aaccctcgcg ggttaccggg tgacggtgtt
      121 tgcaaggcag tatacaaaaa atggccggag tcctttaaga acagtgcaac accagtggga
      181 accgcaaaaa cagttatgtg cggtacgtat ccagtaatcc acgctgttgg accaaacttc
      241 tctaattatt cggagtctga aggggaccgg gaattggcag ctgcctatcg agaagtcgca
      301 aaggaagtaa ctaggctggg agtaaatagt gtagctatac ctctcctctc cacaggtgta
      361 tactcaggag ggaaagacag gctgacccag tcactgaacc acctctttac agccatggac
      421 tcgacggatg cagacgtggt catctactgc cgcgacaaag aatgggagaa gaaaatatct
      481 gaggccatac agatgcggac ccaagtagag ctgctggatg agcacatctc catagactgc
      541 gatattgttc gcgtgcaccc tgacagcagc ttggcaggca gaaaaggata cagcaccacg
      601 gaaggcgcac tgtactcata tctagaaggg acccgttttc atcagacggc tgtggatatg
      661 gcggagatac atactatgtg gccaaagcaa acagaggcca atgagcaagt ctgcctatat
      721 gccctggggg aaagtattga atcgatcagg cagaaatgcc cggtggatga tgcagacgca
      781 tcatctcccc ccaaaactgt cccgtgcctt tgccgttacg ctatgactcc agaacgcgtc
      841 acccggcttc gcatgaacca cgtcacaagc ataattgtgt gttcttcgtt tcccctccca
      901 aagtacaaaa tagaaggagt gcaaaaagtc aaatgctcta aggtaatgct atttgaccac
      961 aacgtgccat cgcgcgtaag tccaagggaa tatagatctt cccaggagtc tgcacaggag
     1021 gcgagtacaa tcacgtcact gacgcatagt caattcgacc taagcgttga tggcgagata
     1081 ctgcccgtcc cgtcagacct ggatgctgac gccccagccc tagaaccagc actagacgac
     1141 ggggcgacac acacgctgcc atccacaacc ggaaaccttg cggccgtgtc tgactgggta
     1201 ataagcaccg tacctgtcgc gccgcccaga agaaggcgag ggagaaacct gactgtgaca
     1261 tgtgacgaga gagaagggaa tataacaccc atggctagcg tccgattctt tagggcagag
     1321 ctgtgtccgg tcgtacaaga aacagcggag acgcgtgaca cagcaatgtc tcttcaggca
     1381 ccaccgagta ccgccacgga accgaatcat ccgccgatct ccttcggagc atcaagcgag
     1441 acgttcccca ttacatttgg ggacttcaac gaaggagaaa tcgaaagctt gtcttctgag
     1501 ccactaactt tcggagactt cttaccagga gaagtggatg acttgacaga cagcgactgg
     1561 tccacgtgct cagacacgga cgacgagtta gactacaaag accatgacgg tgattataaa
     1621 gatcatgata tcgattacaa ggatgacgat gacaagtagt aactcgaggc ggccgc
//