LOCUS pOME0162 1676 bp DNA linear UNA 17-Jan-2014 DEFINITION CHIKV nsP3 early stop; missing 6 last amino acids; Sri Lankan isolate TAGGED; vector pDEST47 COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of the Orfeome project CHIKV= Chickungunya Virus nsP= non-structural protein FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..1659 /cds_type=ORF /note="Length: 1641" /note="Found at strand: positive" /note="Start codon: atg" CDS 701..826 /cds_type=ORF /note="Length: 126" /note="Found at strand: positive" /note="Start codon: atg" Site 1591..1656 /site_type="mutagenized site" /label="3X FLAG tag" Site 1657..1662 /site_type="mutagenized site" /label="double stop" Site join(1663..1668,1663^1664) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 1663^1664" /note="cut_0_on_negative_strand: 1667^1668" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 1663..1676 /site_type="mutagenized site" /label="3' RE tag" Site join(1669..1676,1670^1671) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 1670^1671" /note="cut_0_on_negative_strand: 1674^1675" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccat ggcaccgtcg taccgggtaa aacgcatgga catcgcgaag 61 aacgatgaag agtgcgtagt caacgccgct aaccctcgcg ggttaccggg tgacggtgtt 121 tgcaaggcag tatacaaaaa atggccggag tcctttaaga acagtgcaac accagtggga 181 accgcaaaaa cagttatgtg cggtacgtat ccagtaatcc acgctgttgg accaaacttc 241 tctaattatt cggagtctga aggggaccgg gaattggcag ctgcctatcg agaagtcgca 301 aaggaagtaa ctaggctggg agtaaatagt gtagctatac ctctcctctc cacaggtgta 361 tactcaggag ggaaagacag gctgacccag tcactgaacc acctctttac agccatggac 421 tcgacggatg cagacgtggt catctactgc cgcgacaaag aatgggagaa gaaaatatct 481 gaggccatac agatgcggac ccaagtagag ctgctggatg agcacatctc catagactgc 541 gatattgttc gcgtgcaccc tgacagcagc ttggcaggca gaaaaggata cagcaccacg 601 gaaggcgcac tgtactcata tctagaaggg acccgttttc atcagacggc tgtggatatg 661 gcggagatac atactatgtg gccaaagcaa acagaggcca atgagcaagt ctgcctatat 721 gccctggggg aaagtattga atcgatcagg cagaaatgcc cggtggatga tgcagacgca 781 tcatctcccc ccaaaactgt cccgtgcctt tgccgttacg ctatgactcc agaacgcgtc 841 acccggcttc gcatgaacca cgtcacaagc ataattgtgt gttcttcgtt tcccctccca 901 aagtacaaaa tagaaggagt gcaaaaagtc aaatgctcta aggtaatgct atttgaccac 961 aacgtgccat cgcgcgtaag tccaagggaa tatagatctt cccaggagtc tgcacaggag 1021 gcgagtacaa tcacgtcact gacgcatagt caattcgacc taagcgttga tggcgagata 1081 ctgcccgtcc cgtcagacct ggatgctgac gccccagccc tagaaccagc actagacgac 1141 ggggcgacac acacgctgcc atccacaacc ggaaaccttg cggccgtgtc tgactgggta 1201 ataagcaccg tacctgtcgc gccgcccaga agaaggcgag ggagaaacct gactgtgaca 1261 tgtgacgaga gagaagggaa tataacaccc atggctagcg tccgattctt tagggcagag 1321 ctgtgtccgg tcgtacaaga aacagcggag acgcgtgaca cagcaatgtc tcttcaggca 1381 ccaccgagta ccgccacgga accgaatcat ccgccgatct ccttcggagc atcaagcgag 1441 acgttcccca ttacatttgg ggacttcaac gaaggagaaa tcgaaagctt gtcttctgag 1501 ccactaactt tcggagactt cttaccagga gaagtggatg acttgacaga cagcgactgg 1561 tccacgtgct cagacacgga cgacgagtta gactacaaag accatgacgg tgattataaa 1621 gatcatgata tcgattacaa ggatgacgat gacaagtagt aactcgaggc ggccgc //