LOCUS pOME0164 1940 bp DNA linear UNA 17-Jan-2014 DEFINITION CHIKV nsP4 Sri Lankan isolate TAGGED; vector pDEST47 COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of the Orfeome project CHIKV= Chickungunya Virus nsP= non-structural protein FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site 1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..1923 /cds_type=ORF /note="Length: 1905" /note="Found at strand: positive" /note="Start codon: atg" Site 19..21 /site_type="mutagenized site" /label=start CDS 1467..1613 /cds_type=ORF /note="Length: 147" /note="Found at strand: positive" /note="Start codon: atg" Site 1855..1920 /site_type="mutagenized site" /label="3X FLAG tag" Site 1921..1926 /site_type="mutagenized site" /label="double stop" Site join(1927..1932,1927^1928) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 1927^1928" /note="cut_0_on_negative_strand: 1931^1932" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 1927..1940 /site_type="mutagenized site" /label="3' RE tag" Site join(1933..1940,1934^1935) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 1934^1935" /note="cut_0_on_negative_strand: 1938^1939" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccat gtatatattc tcgtcggaca ccggtccagg tcatttacaa 61 cagaagtcag tacgccagtc agtgctgccg gtgaacaccc tggaggaagt ccacgaggag 121 aagtgttacc cacctaagct ggatgaagca aaggagcaac tattacttaa gaaactccag 181 gagagtgcat ccatggccaa cagaagcagg tatcagtcgc gcaaagtaga aaacatgaaa 241 gcagcaatca tccagagact aaagagaggc tgtagactat acttaatgtc agagacccca 301 aaagtcccta cttaccggac tacatatccg gcgcctgtgt actcgcctcc gatcaacgtt 361 cgattgtcca atcccgagtc cgcagtggca gcatgcaatg agttcttagc tagaaactat 421 ccaactgtct catcatacca aattaccgac gagtatgatg catatctaga catggtggac 481 gggtcggaga gttgcctgga ccgagcgaca ttcaatccgt caaaactcag gagctacccg 541 aaacagcacg cttaccacgc gccctccatc agaagcgctg taccgtcccc attccagaac 601 acactacaga atgtactggc agcagccacg aaaagaaact gcaacgtcac acagatgagg 661 gaattaccca ctttggactc agcagtattc aacgtggagt gtttcaaaaa attcgcatgc 721 aaccaagaat actgggaaga atttgctgcc agccctatta ggataacaac tgagaattta 781 gcaacctatg ttactaaact aaaagggcca aaagcagcag cgctattcgc aaaaacccat 841 aatctactgc cactacagga agtaccaatg gataggttca cagtagatat gaaaagggac 901 gtgaaggtga ctcctggtac aaagcataca gaggaaagac ctaaggtgca ggttatacag 961 gcggctgaac ccttggcgac agcataccta tgtgggattc acagagagct ggttaggagg 1021 ctgaacgccg tcctcctacc caatgtacat acactatttg acatgtctgc cgaggatttc 1081 gatgccatca tagccgcaca ctttaagcca ggagacactg ttttggaaac ggacatagcc 1141 tcctttgata agagccaaga tgattcactt gcgcttactg ctttgatgct gttagaggat 1201 ttaggggtgg atcactccct gctggacttg atagaggctg ctttcggaga gatttccagc 1261 tgtcacctac cgacaggtac gcgcttcaag ttcggcgcca tgatgaaatc aggtatgttc 1321 ctaactctgt tcgtcaacac attgttaaac atcaccatcg ccagccgagt gctggaagat 1381 cgtctgacaa aatccgcgtg cgcggccttc atcggcgacg acaacataat acatggagtc 1441 gtctccgatg aattgatggc agccagatgt gccacttgga tgaacatgga agtgaagatc 1501 atagatgcag ttgtatcctt gaaagcccct tacttttgtg gagggtttat actgcacgat 1561 actgtgacag gaacagcttg cagagtggca gacccgctaa aaaggctttt taaactgggc 1621 aaaccgctag cggcaggtga cgaacaagat gaagatagaa gacgagcgct ggctgacgaa 1681 gtgatcagat ggcaacgaac agggctaatt gatgagctgg agaaagcggt atactctagg 1741 tacgaagtgc agggtatatc agttgtggta atgtccatgg ccacctttgc aagctccaga 1801 tccaatttcg agaagctcag aggacccgtc ataactttgt acggcggtcc taaagactac 1861 aaagaccatg acggtgatta taaagatcat gatatcgatt acaaggatga cgatgacaag 1921 tagtaactcg aggcggccgc //