LOCUS pOME0174 383 bp DNA linear UNA 27-Jan-2014 DEFINITION A/Anhui H7N9 NS Hypo #2 TAGGED; vector pDEST47 SOURCE UNC166-4 (A/Anhui H7N9 NS Hypo #2 TAGGED) COMMENT Plasmid synthesized by BioBasic Sequence verified via Sanger sequencing through Genewiz pOME= plasmid of the Orfeome project A/Anhui H7N9 NS Hypo= Influenza type A/ Anhui H7N9/Hypothetical FEATURES Location/Qualifiers Site join(1..6,1^2) /site_type="restriction site" /note="Name: SalI" /note="Pattern: gtcgac" /note="cut_0_on_positive_strand: 1^2" /note="cut_0_on_negative_strand: 5^6" /note="inhibited_by: 5': N6-methyladenosine" /note="star_activity: yes" /note="site_type: other" /note="restriction site" /label=SalI Site <1..12 /site_type="mutagenized site" /label="5' RE tag" Site join(7..12,11^12) /site_type="restriction site" /note="Name: KpnI" /note="Pattern: ggtacc" /note="cut_0_on_positive_strand: 11^12" /note="cut_0_on_negative_strand: 7^8" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=KpnI Site 13..18 /site_type="mutagenized site" /label="Kozak sequence" CDS 19..366 /cds_type=ORF /note="Length: 348" /note="Found at strand: positive" /note="Start codon: ATG" Site 298..363 /site_type="mutagenized site" /label="3X FLAG tag" Site 364..369 /site_type="mutagenized site" /label="double stop" Site join(370..375,370^371) /site_type="restriction site" /note="Name: XhoI" /note="Pattern: ctcgag" /note="cut_0_on_positive_strand: 370^371" /note="cut_0_on_negative_strand: 374^375" /note="inhibited_by: 5': N6-methyladenosine" /note="site_type: other" /note="restriction site" /label=XhoI Site 370..383 /site_type="mutagenized site" /label="3' RE tag" Site join(376..383,377^378) /site_type="restriction site" /note="Name: NotI" /note="Pattern: gcggccgc" /note="cut_0_on_positive_strand: 377^378" /note="cut_0_on_negative_strand: 381^382" /note="inhibited_by: 5': N4-methylcytosine" /note="site_type: other" /note="restriction site" /label=NotI ORIGIN 1 gtcgacggta ccgccaccAT GAGGATCTCA ATTGCATTTT TGACATCCTT GTCAGTATGT 61 CCTGGAAGAG AAGGTAATGG TGAGATTTCG CCTACAATTG CTCCTTCTTC CGTAAAAGCT 121 CTAAGTAGTA TCAGGGCTTC AAGCCGATTG AAAATCACAC TGAAATTTGC TTTCAATGTG 181 ATGTTTTTGT CCACTATTGC TTGGTCCATT CTAATGCATA GGGACCCTGT TATTTTCTGT 241 TTGGGAATGA GCATTAACCA ATCTCTTGAC ATTTCTTCAA GAGTCATGTC AGTTAGAgac 301 tacaaagacc atgacggtga ttataaagat catgatatcg attacaagga tgacgatgac 361 aagtagtaac tcgaggcggc cgc //