LOCUS       pOME0174       383 bp    DNA     linear   UNA 27-Jan-2014
DEFINITION  A/Anhui H7N9 NS Hypo #2 TAGGED; vector pDEST47
SOURCE      UNC166-4 (A/Anhui H7N9 NS Hypo #2 TAGGED)
COMMENT     Plasmid synthesized by BioBasic
            Sequence verified via Sanger sequencing through Genewiz
            pOME= plasmid of the Orfeome project
            A/Anhui H7N9 NS Hypo= Influenza type A/ Anhui H7N9/Hypothetical 
FEATURES             Location/Qualifiers
     Site            join(1..6,1^2)
                     /site_type="restriction site"
                     /note="Name: SalI"
                     /note="Pattern: gtcgac"
                     /note="cut_0_on_positive_strand: 1^2"
                     /note="cut_0_on_negative_strand: 5^6"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=SalI
     Site            <1..12
                     /site_type="mutagenized site"
                     /label="5' RE tag"
     Site            join(7..12,11^12)
                     /site_type="restriction site"
                     /note="Name: KpnI"
                     /note="Pattern: ggtacc"
                     /note="cut_0_on_positive_strand: 11^12"
                     /note="cut_0_on_negative_strand: 7^8"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=KpnI
     Site            13..18
                     /site_type="mutagenized site"
                     /label="Kozak sequence"
     CDS             19..366
                     /cds_type=ORF
                     /note="Length: 348"
                     /note="Found at strand: positive"
                     /note="Start codon: ATG"
     Site            298..363
                     /site_type="mutagenized site"
                     /label="3X FLAG tag"
     Site            364..369
                     /site_type="mutagenized site"
                     /label="double stop"
     Site            join(370..375,370^371)
                     /site_type="restriction site"
                     /note="Name: XhoI"
                     /note="Pattern: ctcgag"
                     /note="cut_0_on_positive_strand: 370^371"
                     /note="cut_0_on_negative_strand: 374^375"
                     /note="inhibited_by: 5': N6-methyladenosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=XhoI
     Site            370..383
                     /site_type="mutagenized site"
                     /label="3' RE tag"
     Site            join(376..383,377^378)
                     /site_type="restriction site"
                     /note="Name: NotI"
                     /note="Pattern: gcggccgc"
                     /note="cut_0_on_positive_strand: 377^378"
                     /note="cut_0_on_negative_strand: 381^382"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=NotI
ORIGIN
        1 gtcgacggta ccgccaccAT GAGGATCTCA ATTGCATTTT TGACATCCTT GTCAGTATGT
       61 CCTGGAAGAG AAGGTAATGG TGAGATTTCG CCTACAATTG CTCCTTCTTC CGTAAAAGCT
      121 CTAAGTAGTA TCAGGGCTTC AAGCCGATTG AAAATCACAC TGAAATTTGC TTTCAATGTG
      181 ATGTTTTTGT CCACTATTGC TTGGTCCATT CTAATGCATA GGGACCCTGT TATTTTCTGT
      241 TTGGGAATGA GCATTAACCA ATCTCTTGAC ATTTCTTCAA GAGTCATGTC AGTTAGAgac
      301 tacaaagacc atgacggtga ttataaagat catgatatcg attacaagga tgacgatgac
      361 aagtagtaac tcgaggcggc cgc
//